[Histonet] cement in bone specimen

[Jack Ratliff]

Peggy,

 

If the cement you mention is or similar to PMMA, then you will not be able to cut thin sections using the sliding/sledge microtome. If I was you, I would not decal it at all as it will only make the bone less dense than the cement and will definitely waste your time if you embed it in paraffin to try and cut later. Your best and only chance at success here is to embed undemineralized in resin and cut thick or ground sections that can be polished to a desired thickness.

 

If you would like to discuss this further, please give me a call at 317-281-1975.

 

Best,

 

Jack Ratliff

 


 
> Date: Thu, 6 Aug 2009 15:43:16 -0400
> From: MDiCarlo <@t> KaleidaHealth.Org
> To: histonet <@t> pathology.swmed.edu
> CC: 
> Subject: [Histonet] cement in bone specimen
> 
> Histonetters,
> 
> 
> 
> We sliced a humerus bone on a band saw which was filled with cement from
> a previous surgery and my boss would like me to try to cut it on a
> sliding/sledge microtome. (The knife remains stationary) Is this
> possible to do? Presently, it is fixing and then I will decal it but
> I'm wondering if I'll be wasting my time and effort. 
> 
> 
> 
> I would appreciate anyone's experience with this.
> 
> 
> 
> Thank you.
> 
> 
> 
> Peggy DiCarlo HT (ASCP)
> 
> Orthopedic Bone Lab
> 
> Buffalo General Hospital
> 
> Buffalo, NY 14203
> 
> 716-859-1293
> 
> 
> 
> 
> 
> 2009 Best Places to Work Winner 
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