[Histonet] working with mouse liver...

Michelle MacVeigh-Aloni macveigh <@t> usc.edu
Tue Aug 4 18:20:53 CDT 2009

Hi all,

We have a small liver core and we are having problem with the look/morphology of the liver. It looks like large part of the cytoplasm of the liver cells is missing. The normal mice are the worst. (It is not fat that is dissolved.)
We use commercially made 10% NBF and tried fixing for different length of time with no luck.

The processing time is 1 hour in every station.

Did anybody working with liver has any suggestions?


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