[Histonet] canine bone, EDTA

[Vicki Kalscheur]

Peggy,  If your microtome will hold a sharp D profile or a tungsten carbide blade,  you might be able to get consistent sections.  I find that EDTA decalcification hardens the tissue ( as if it wasn't hard enough already).  Also, extending the times for the paraffin infiltration is helpful and soaking the block in icy/soapy detergent in the freezer works on some samples.

~ VIcki