[Histonet] Cracked or shattered LN sections

[Rene J Buesa]

Shattering is not produced at the embedding section. It is caused during processing specially because of improper dehydration. If the LN are too thick to begin with, it will also contribute to improper dehydration and as a consequence poor infiltration.
René J.

--- On Mon, 4/27/09, Greg Dobbin <gvdobbin <@t> ihis.org> wrote:

From: Greg Dobbin <gvdobbin <@t> ihis.org>
Subject: [Histonet] Cracked or shattered LN sections
To: Histonet <@t> lists.utsouthwestern.edu
Date: Monday, April 27, 2009, 3:04 PM

Hi Folks,
Some lymph node sections (usually bigger ones-maybe almost as big as a
dime) have a shattered appearance on the block surface. Subsequently the
sections look like shattered glass or a mosaic of tissue pieces making
up the whole. I think this is happening at the embedding station from
trying to make sure that the tissue is flat in the mold (ie pressing the
tissue down). Has anyone else out there have any similar experience with
this problem? Is the solution as simple as cutting smaller pieces into
the cassette at the time of trimming?
Thanks.
Greg

Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE    C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

"I find that the harder I work, the 
more luck I seem to have."
- Thomas Jefferson


-------------------------
Statement of Confidentiality
This message (including attachments) may contain confidential or privileged
information intended for a specific individual or organization. If you have
received this communication in error, please notify the sender immediately. If
you are not the intended recipient, you are not authorized to use, disclose,
distribute, copy, print or rely on this email, and should promptly delete this
email from your entire computer system.

D?claration de confidentialit?
Le pr?sent message (y compris les annexes) peut contenir des renseignements
confidentiels ? l'intention d'une personne ou d'un organisme
particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en
informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire
pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer,
copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez
l'effacer imm?diatement de votre syst?me informatique.
-------------------------


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet