[Histonet] Cracked or shattered LN sections

Rene J Buesa rjbuesa <@t> yahoo.com
Tue Apr 28 08:32:23 CDT 2009


Shattering is not produced at the embedding section. It is caused during processing specially because of improper dehydration. If the LN are too thick to begin with, it will also contribute to improper dehydration and as a consequence poor infiltration.
René J.

--- On Mon, 4/27/09, Greg Dobbin <gvdobbin <@t> ihis.org> wrote:

From: Greg Dobbin <gvdobbin <@t> ihis.org>
Subject: [Histonet] Cracked or shattered LN sections
To: Histonet <@t> lists.utsouthwestern.edu
Date: Monday, April 27, 2009, 3:04 PM

Hi Folks,
Some lymph node sections (usually bigger ones-maybe almost as big as a
dime) have a shattered appearance on the block surface. Subsequently the
sections look like shattered glass or a mosaic of tissue pieces making
up the whole. I think this is happening at the embedding station from
trying to make sure that the tissue is flat in the mold (ie pressing the
tissue down). Has anyone else out there have any similar experience with
this problem? Is the solution as simple as cutting smaller pieces into
the cassette at the time of trimming?
Thanks.
Greg

Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE    C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

"I find that the harder I work, the 
more luck I seem to have."
- Thomas Jefferson


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