[Histonet] Re: Embedding Issues

[Shelly Coker]

I am not sure if you are referring to bubble between the tissue and the bottom of the molds, or bubbles causing holes between the tissue and the cassette, causing the block to "collapse".  The previous would probably have something to do with the paraffin feeding into the block, perhaps there is a leak in the system causing air bubbles in the paraffin.  As for the latter, we have experienced this with the cassettes that we use, and have found that 3-4 good hard taps on the top of the cassette after filling with paraffin has helped.  Also, we do have to be sure that the initial fill of paraffin is just above the lip of the mold, so that when we set the cassette on top, there is a small amount of paraffin in the cassette already.  These two steps have all but eliminated the air bubbles between the tissue and the cassette (blocks collapsing). 
 
Good luck and hope this helps,
Shelly
--- On Mon, 4/27/09, histonet-request <@t> lists.utsouthwestern.edu <histonet-request <@t> lists.utsouthwestern.edu> wrote:


From: histonet-request <@t> lists.utsouthwestern.edu <histonet-request <@t> lists.utsouthwestern.edu>
Subject: Histonet Digest, Vol 65, Issue 45
To: histonet <@t> lists.utsouthwestern.edu
Date: Monday, April 27, 2009, 1:57 PM


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Today's Topics:

   1. help (Dertien, Janet)
   2. Re: help (Rene J Buesa)
   3. RE Finding Iron in Decalcified Sections (Farish, Craig)
   4. Doublecortin, Ki67, Caspase-3: need antigen retrieval ? (TF)
   5. RE Megacassettes (Farish, Craig)
   6. RE: Histonet Digest, Vol 65, Issue 44 (Stella_quan <@t> DNAR.com)
   7. AMMONIUM HYDROXIDE (Phyllis Thaxton)
   8. RE: AMMONIUM HYDROXIDE (Sebree Linda A)
   9. RE: AMMONIUM HYDROXIDE (Bartlett, Jeanine (CDC/CCID/NCZVED))
  10. AW: [Histonet] help (Gudrun Lang)
  11. HT exam (MaryAnn Dixon)
  12. Re: HT exam (Pamela Marcum)
  13. Sensitivity and Specificity of Antibodies for IHC (Jessica Piche)
  14. Re: HT exam (stephanie.d.rivera <@t> gsk.com)
  15. Embedding issues (Sharon.Davis-Devine)
  16. breast tissue (Caleri, Kathleen)
  17. RE: haemotol autostainer? (Edwards, R.E.)
  18. Re: AMMONIUM HYDROXIDE (Rene J Buesa)

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