[Histonet] different microtome?

[Pamela Marcum]

Peggy, 



We are cutting decal in paraffin and non-decal bone in MMA on Leica RM2255 microtomes almost daily.  The motor is strong enough for the MMA and we have great success using both manual and motorized sectioning for decal bone.  We do large pieces of sheep spine for both.  The sections have always been even for just picking up a few sections or serial sections.  Hope this helps. 



Pam Marcum 

UPENN Vet Sch 

New Bolton Center 



----- Original Message ----- 
From: "Margaret DiCarlo" <MDiCarlo <@t> KaleidaHealth.Org> 
To: histonet <@t> pathology.swmed.edu 
Sent: Monday, April 27, 2009 3:16:39 PM GMT -05:00 US/Canada Eastern 
Subject: [Histonet] different microtome? 

Hello Everyone, 

  

I am attempting to cut decaled (with EDTA) dog bone tissue which is 
attached to a partial tooth, manually processed and embedded in Tissue 
Prep 2 paraffin.  I have cut one block but have only gotten thick and 
thin sections.  I am currently using a Microm HM 325 using high profile 
disposable blades.  My boss wanted me to find out if there is a better 
microtome out there that could cut this tissue serially.  Are there any? 

  

Thank you. 

  

Peggy DiCarlo HT (ASCP) 

Orthopedic Bone Lab 

Buffalo General Hospital 

Buffalo, NY  14203 

716-859-1293 

  



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