[Histonet] TRAP staining for osteoclasts

[nicole.rahde <@t> terra.com.br]

 Hi!
 I am having trouble with TRAP staining on formalin fixed, paraffin
embedded, formic acid
 decalcified rat bone. The positive control (wich was not
decalcified) showed beautifully marked osteoclasts, but the rat bone
did not at all.
 Would the formic acid used in decalcification impair the reaction?
 Does anyone know something I could do to to make the reaction work
on these specimens?
 Thanks a lot!
 Best regards,
 Nicole Rahde 
 DDS, MSc, PhD student, Oral Medicine, PUC-RS
 Porto Alegre, Brazil.