[Histonet] Control slides for Oil Red O
gagnone <@t> KGH.KARI.NET
Fri Apr 24 14:52:41 CDT 2009
I was always of the opinion that since a delipidized frozen section, treated with toluene/methanol, run in parallel with the patient slide constituted adequate control. Recently though, I've mellowed and realized that only shows a difference in staining between the two slides, not the effectiveness of your stain. Using a piece of fat for a control slide confirms microscopically what we can confirm macroscopically...yep, it's a piece of fat.
The adrenal and skin ideas sound better to me than using a piece of breast/fatty tissue.
The era of performing staining methods without controls seems to be at an end, which isn't necessarily a bad thing.
Eric Gagnon MLT
Kingston General Hospital
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