[Histonet] RE: Is this ok for Histonet?

Fri Apr 24 14:06:41 CDT 2009

Is this OK for the Histonet:

ATTENTION!:VA, PA, MD, NJ, DE, W. VA, and DC
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-Antibody Panels in the Anatomic Laboratory
-Multiplex Stains for the Anatomic Pathology Laboratory  

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: Friday, April 24, 2009 1:01 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 65, Issue 42

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Today's Topics:

   1. RE: ground meat samples (Charles, Roger)
   2. RE: ground meat samples (Rittman, Barry R)
   3. Ground meat processing (Breeden, Sara)
   4. RE: levamisole (Shakun Aswani)
   5. Re: EM Knife (Geoff McAuliffe)
   6. new vs used microtomes(regular rotary) (Madary, Joseph)
   7. Grossing (Schaundra Walton)
   8. Fatty Breast Tissue processing (Bull, Jennifer L.)
   9. Herpes (Mark Tarango)
  10. ground meat processing (Dolores_Fischer <@t> baxter.com)
  11. commercial control slides (Steven Joy)
  12. Re: Myeloperoxidase Antibody (TF)
  13. RE: commercial control slides (Ingles Claire )
  14. AW: [Histonet] ground meat samples (Gudrun Lang)
  15. ground meat samples (Stephen Peters M.D.)
  16. Re: commercial control slides (Joe Nocito)
  17. Re: Grossing (Joe Nocito)
  18. RE: commercial control slides (Lee & Peggy Wenk)
  19. non-specific fluorescence in artery (Sarah Tarran)
  20. Doublecortin staining with Abcam antibody (TF)
  21. Opening In Texas (Alyssa Peterson)
  22. Mega cassettes (Ian Montgomery)
  23. RE: Doublecortin staining with Abcam antibody (Montina Van Meter)
  24. Re: Mega cassettes (Amy Porter)
  25. paraffin embedding (Ryan McAdams)
  26. benzene/paraffin (Maruska, Ann)

----------------------------------------------------------------------

Message: 1
Date: Thu, 23 Apr 2009 13:20:37 -0400
From: "Charles, Roger" <rcharles <@t> state.pa.us>
Subject: [Histonet] RE: ground meat samples
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<3809C163DC1DA54AA534B3C7794D07B632A1C80A45 <@t> ENHBGMBX01.PA.LCL>
Content-Type: text/plain; charset="us-ascii"

Sorry Histonetter's
But it is my Friday and wouldn't this method give new meaning to the term "sausage block." :) I wish Margaret all the luck in her quest and if it was me, I would try either or both methods.

Roger Charles
Microbiologist
Pennsylvania Veterinary Laboratory
2305 N Cameron St
Harrisburg, PA 17110
717-787-8808
rcharles <@t> state.pa.us

No trees were hurt in the sending of this email, However many electrons were severely inconvenienced!

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Perry, Margaret
Sent: Thursday, April 23, 2009 12:53 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] ground meat samples

We will be doing IHC on ground pork and would like tips on how to process and embed the samples.  Can you leave the processed sample in a paper biopsy bag and embed both the meat and the paper?  Will cutting the paper make the blade dull to quickly?  Is there anything else we can use to hold the tissue together?  We had thought to maybe use pig intestine and pack the meat into it to make it cut better.
Thank you in advance for your help.  Histonet has helped me out many times and I greatly appreciate it.

Margaret Perry HT (ASCP)
IHC Lab Manager Veterinary Science
Animal Disease Research and Diagnostic Lab
South Dakota State University
Box 2175 North Campus Drive
Brookings SD 57007

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------------------------------

Message: 2
Date: Thu, 23 Apr 2009 12:48:17 -0500
From: "Rittman, Barry R" <Barry.R.Rittman <@t> uth.tmc.edu>
Subject: [Histonet] RE: ground meat samples
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<75A0543E23D3A7458012D9E02EDBEC0002E9248A46 <@t> UTHCMS1.uthouston.edu>
Content-Type: text/plain; charset="us-ascii"

Roger
If I were you I would compress this into a block before fixation.
Can use paper up to wax but would not use paper covering in the embedded block it will make cutting too difficult.
Alternative is to use sausage skin as the covering and process, embed and cut with this.
Barry

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Charles, Roger
Sent: Thursday, April 23, 2009 12:21 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: ground meat samples

Sorry Histonetter's
But it is my Friday and wouldn't this method give new meaning to the term "sausage block." :) I wish Margaret all the luck in her quest and if it was me, I would try either or both methods.

Roger Charles
Microbiologist
Pennsylvania Veterinary Laboratory
2305 N Cameron St
Harrisburg, PA 17110
717-787-8808
rcharles <@t> state.pa.us

No trees were hurt in the sending of this email, However many electrons were severely inconvenienced!

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Perry, Margaret
Sent: Thursday, April 23, 2009 12:53 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] ground meat samples

We will be doing IHC on ground pork and would like tips on how to process and embed the samples.  Can you leave the processed sample in a paper biopsy bag and embed both the meat and the paper?  Will cutting the paper make the blade dull to quickly?  Is there anything else we can use to hold the tissue together?  We had thought to maybe use pig intestine and pack the meat into it to make it cut better.
Thank you in advance for your help.  Histonet has helped me out many times and I greatly appreciate it.

Margaret Perry HT (ASCP)
IHC Lab Manager Veterinary Science
Animal Disease Research and Diagnostic Lab
South Dakota State University
Box 2175 North Campus Drive
Brookings SD 57007

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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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------------------------------

Message: 3
Date: Thu, 23 Apr 2009 11:50:06 -0600
From: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
Subject: [Histonet] Ground meat processing
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<4D14F0FC9316DD41972D5F03C070908B017E682B <@t> nmdamailsvr.nmda.ad.nmsu.edu>

Content-Type: text/plain;	charset="us-ascii"

What would be wrong with just submitting the ground sample as one would
with a curetting or similar - that is if the pieces are small, submit
them for processing in a lens paper packet or one of those paper histo
bags, then scrape the pieces off the paper and embed into paraffin as
usual?  Perhaps I'm thrown off a bit by the part about embedding the
paper along with the tissue? Do you have some special requirement for
processing for IHC that makes it necessary to consider embedding the
paper? 

Sally Breeden, HT(ASCP)

NM Dept. of Agriculture

Veterinary Diagnostic Services

PO Box 4700

Albuquerque, NM  87106

505-841-2576

------------------------------

Message: 4
Date: Thu, 23 Apr 2009 10:52:15 -0700
From: "Shakun Aswani" <Shakun.Aswani <@t> acologix.com>
Subject: RE: [Histonet] levamisole
To: "anjan kumar" <drvet_anjan <@t> hotmail.com>,	"triple immunohistochem"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<777AB0DE519C8E46A6220E2287C5BAD301B34E9E <@t> EXCHANGE.acologix.com>
Content-Type: text/plain;	charset="US-ASCII"

Hi Anjan,

Here is the molecular weight of the levamisole, from this you can
calculate the 125mM

Levamisole Analytical Information

* Synonym: leva (l)-Tetramisole

* Chemical Name: (6S)-2,3,5,6-Tetrahydro-6-phenylimidazo[2,1-b]thiazole

* Chemical Formula: C11H12N2S*HCl

* Molecular Weight: 204.3

* Formula Weight: 240.8

* pKa: 8.0

* Levamisole is extracted using a basic liquid-liquid chlorobutane
extraction with an acid back extraction.

* Levamisole is easily detected on the GC/NPD and GC/MS.

* Elution Order: Doxylamine, LEVAMISOLE, Chlorpheniramine

I hope this will help you

Regards,

Shakun

Note:

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of anjan
kumar
Sent: Wednesday, April 22, 2009 1:42 PM
To: triple immunohistochem
Subject: [Histonet] levamisole

hi everyone,

                  can anyone tell me how to prepare 125mM of levamisole.

Dr. Anjan Kumar.K.R

M.V.Sc Scholar

Dept. of Veterinary Pathology

Madras Veterinary College

Chennai-7

India

email: drvet_anjan <@t> hotmail.com

Phone: +91-9940475801

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------------------------------

Message: 5
Date: Thu, 23 Apr 2009 13:56:57 -0400
From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
Subject: Re: [Histonet] EM Knife
To: Stella Mireles <estellamireles <@t> gmail.com>
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <49F0ABE9.5050704 <@t> umdnj.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

Hi Stella:

All of our knives are Diatome and I have no complaints. On the other 
hand, I have been doing business with Pelco for many years with no 
problems ever. It seems unlilely that they are making their own knives 
so they are probably buying and rebranding. You will probably be fine 
with either.

Geoff

Stella Mireles wrote:
> All EM Techs.  Which Ultra knife is better.  Diatome or Pelco?  Is there any
> other company that I should consider.  I plan to purchase an Ultra and a
> Histo knife.
> Thank You
> Stella
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>   

-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff <@t> umdnj.edu
**********************************************

------------------------------

Message: 6
Date: Thu, 23 Apr 2009 14:08:05 -0400
From: "Madary, Joseph" <MadaryJ <@t> MedImmune.com>
Subject: [Histonet] new vs used microtomes(regular rotary)
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<E5B1C3D1305EFD4C9CA7F63094135F5E01E3915F <@t> MD1EV002.medimmune.com>
Content-Type: text/plain;	charset="us-ascii"

I bought a couple of Microms and so far they have been great.  Wondering
if anyone has purchased a new rotary microtome recently for a decent
price that they like.  I have bought a couple of used as well many years
ago from a guy I trusted no longer in the business.  Any suggestions
there? I have purchased used equipment from IMEB and BelAir both have
been fine.  Just checking my options here.  I have an RMS that I never
really bothered using that has electronic advance/retract that works
fine but I am a little old school and that little feature is more
annoying where some folks love it.  If there are any vendors out there
who deal in used equipment and would give me something on a trade in,
that might make me consider you over someone else.

To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary.  This communication is expected to be read and/or used only by the individual(s) for whom it is intended.  If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose.  Thank you for your cooperation.

------------------------------

Message: 7
Date: Thu, 23 Apr 2009 11:25:22 -0700 (PDT)
From: Schaundra Walton <schaundrawalton <@t> yahoo.com>
Subject: [Histonet] Grossing
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <435583.27358.qm <@t> web58908.mail.re1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

We are evaluating some of our grossing procedures and cut off times and I was wondering what other people are doing.

Who does your grossing, pathologist, PA, or other qualified individuals?
Who does you accessioning, histotechs, support staff, or other?
What is your cut off time for grossing tissues?

Right now our pathologists gross all tissues, the histotechs accession, and grossing is done up until 6pm (which is when the processor begins).

Thanks for the info!

-Schaundra Walton HTL(ASCP)
Histology Supervisor
Swedish American Hospital
Rockford, IL

------------------------------

Message: 8
Date: Thu, 23 Apr 2009 11:27:07 -0700
From: "Bull, Jennifer L." <Jennifer.Bull <@t> northwestpathology.com>
Subject: [Histonet] Fatty Breast Tissue processing
To: "'histonet <@t> lists.utsouthwestern.edu'"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<85760CECEC18444BB95F26D5E88DAEAA223F261C25 <@t> hinet2.hinet.org>
Content-Type: text/plain; charset="us-ascii"

Is anyone willing to share their protocol for processing fatty breast tissue? It is difficult to find any documentation or processes that work really well. Thank you in advance, you have all been extremely helpful to me!

Jennifer Bull
Histology Supervisor
jennifer.bull <@t> nwpathology.com

------------------------------

Message: 9
Date: Thu, 23 Apr 2009 11:36:23 -0700
From: Mark Tarango <marktarango <@t> gmail.com>
Subject: [Histonet] Herpes
To: "histonet <@t> lists.utsouthwestern.edu"
	<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<5b6eb13e0904231136j4bccbb31i55c757c86e053378 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Does anyone have a herpes control for which they'd be willing to trade?  I
can trade just about any tumor or control material you might need....

thanks

Mark Tarango

------------------------------

Message: 10
Date: Thu, 23 Apr 2009 13:37:01 -0500
From: Dolores_Fischer <@t> baxter.com
Subject: [Histonet] ground meat processing
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<OF6878747E.ECA4548D-ON862575A1.0065B6B0-862575A1.00664468 <@t> baxter.com>
Content-Type: text/plain; charset="us-ascii"

I agree with Sally, why not just process as a curretting by loosely placing
pieces of ground meat in a cassette and embed in an appropriate sized mold
for your IHC needs.   Paper will shred, it does not section well, if at
all.

Dolores Fischer

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Message: 11
Date: Thu, 23 Apr 2009 12:38:58 -0600
From: "Steven Joy" <Steven.Joy <@t> capitalhealth.ca>
Subject: [Histonet] commercial control slides
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<1101A09566B25D4BB3889A44FDC1E7040A9B18 <@t> uantexchg03.capitalhealth.ca>
Content-Type: text/plain;	charset="US-ASCII"

Can anyone recommend a brand or line of commercial control slides for oil red O?

Thanks,
Steve Joy, BSc. MLT
Research and Development Technologist
5B2.03 Anatomical Pathology
University of Alberta Hospital
8440-112 st
Edmonton Ab
T6G 2B7

Phone: (780) 407-8015
Fax: (780) 407-3009
Email: steven.joy <@t> capitalhealth.ca

This communication is intended for the sole use of the recipient to which it was addressed and may contain confidential, personal or privileged information. Please contact the sender immediately if you are not the intended recipient of this information and do not copy, distribute or take action relying on it. Any communication received in error, or subsequent reply, should be deleted or destroyed. Thank you.

------------------------------

Message: 12
Date: Fri, 24 Apr 2009 02:44:12 +0800
From: "TF" <tifei <@t> foxmail.com>
Subject: Re: [Histonet] Myeloperoxidase Antibody
To: "Smith Wanda" <Wanda.Smith <@t> HCAhealthcare.com>,
	"histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <200904240244042496604 <@t> foxmail.com>
Content-Type: text/plain;	charset="gb2312"

Hi, we use 
Rb a Hu Myeloperoxidase  
Dakocytomat 
A039829 
It works great on rat tissue!

2009-04-24 

TF 

·¢¼þÈË£º Smith Wanda 
·¢ËÍÊ±¼ä£º 2009-04-23  03:16:36 
ÊÕ¼þÈË£º histonet <@t> lists.utsouthwestern.edu 
³ËÍ£º 
Ö÷Ìâ£º [Histonet] Myeloperoxidase Antibody 

Good Afternoon Everyone,
Where do you recommend ordering the pre-dilute antibody Myeloperoxidase MPO Ab-2?  Biocare and Lab Vision have both discontinued carrying it.  Please help!!!!!
Thanks,
Wanda
WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax
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Message: 13
Date: Thu, 23 Apr 2009 13:52:08 -0500
From: "Ingles Claire " <CIngles <@t> uwhealth.org>
Subject: RE: [Histonet] commercial control slides
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<F2F030053F9B7345831BED293A6D57E109A6C7 <@t> UWHC-MAIL01.uwhis.hosp.wisc.edu>

Content-Type: text/plain;	charset="iso-8859-1"

Steve: 
I would think of keeping a frozen block of fatty tissue around and cutting fresh control when you do the stain or throw some precut slides in the freezer after fixation, as the specimen needs to be fresh frozen anyway and cannot come in contact with any alcohols. Skin is an OK tissue, as the sebaceous glands as well as the subdermal fat lights up. I would think it would be pretty difficult, if not impossible to ship slides that need to be kept frozen to prevent staining sensitivity degradation. I can only think of shipping them immersed in formalin? 
Claire

Can anyone recommend a brand or line of commercial control slides for oil red O?

Thanks,
Steve Joy, BSc. MLT
Research and Development Technologist
5B2.03 Anatomical Pathology
University of Alberta Hospital
8440-112 st
Edmonton Ab
T6G 2B7

Phone: (780) 407-8015
Fax: (780) 407-3009
Email: steven.joy <@t> capitalhealth.ca

This communication is intended for the sole use of the recipient to which it was addressed and may contain confidential, personal or privileged information. Please contact the sender immediately if you are not the intended recipient of this information and do not copy, distribute or take action relying on it. Any communication received in error, or subsequent reply, should be deleted or destroyed. Thank you.

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------------------------------

Message: 14
Date: Thu, 23 Apr 2009 22:03:16 +0200
From: "Gudrun Lang" <gu.lang <@t> gmx.at>
Subject: AW: [Histonet] ground meat samples
To: "'Perry, Margaret'" <Margaret.Perry <@t> sdstate.edu>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <2696E9BF0D3D4D3681AAC57D3B6150E1 <@t> dielangs.at>
Content-Type: text/plain;	charset="iso-8859-1"

I think producing a "cytoblock" with histogel or similar could help. 
You can also take citratplasma and coagulate it to a small block with the
meat-pieces inside.
Gudrun

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Perry,
Margaret
Gesendet: Donnerstag, 23. April 2009 18:53
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] ground meat samples

We will be doing IHC on ground pork and would like tips on how to process
and embed the samples.  Can you leave the processed sample in a paper biopsy
bag and embed both the meat and the paper?  Will cutting the paper make the
blade dull to quickly?  Is there anything else we can use to hold the tissue
together?  We had thought to maybe use pig intestine and pack the meat into
it to make it cut better.
Thank you in advance for your help.  Histonet has helped me out many times
and I greatly appreciate it.

Margaret Perry HT (ASCP)
IHC Lab Manager Veterinary Science
Animal Disease Research and Diagnostic Lab
South Dakota State University
Box 2175 North Campus Drive
Brookings SD 57007

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------------------------------

Message: 15
Date: Thu, 23 Apr 2009 16:47:20 -0700 (PDT)
From: "Stephen Peters M.D." <petepath <@t> yahoo.com>
Subject: [Histonet] ground meat samples
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <688810.59118.qm <@t> web45101.mail.sp1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

I thing handling it like a curretting wrapped in lens paper or the sausage ideas will both work well. I will add make sure your tissue is no more 
than 3 mm thick when you process it. Probably better to be a bit thinner. I imagine there will be some beef fat in there and assume it will need
 good penetration.

Stephen Peters M.D. 
Pathology Innovations, LLC 
410 Old Mill Lane, 
Wyckoff, NJ 07481 
Phone and fax 201 847 7600 
www.pathologyinnovations.com 

------------------------------

Message: 16
Date: Thu, 23 Apr 2009 19:39:12 -0500
From: "Joe Nocito" <jnocito <@t> satx.rr.com>
Subject: Re: [Histonet] commercial control slides
To: "Steven Joy" <Steven.Joy <@t> capitalhealth.ca>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <3569DFB947964FD2B2054ABF331AB343 <@t> JoePC>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
	reply-type=original

this is hard to do because of the frozen section requirement. However, we 
make smears out of mayonnaise when we have Oil Red O stain and it works 
well. Good luck

JTT
----- Original Message ----- 
From: "Steven Joy" <Steven.Joy <@t> capitalhealth.ca>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, April 23, 2009 1:38 PM
Subject: [Histonet] commercial control slides

Can anyone recommend a brand or line of commercial control slides for oil 
red O?

Thanks,
Steve Joy, BSc. MLT
Research and Development Technologist
5B2.03 Anatomical Pathology
University of Alberta Hospital
8440-112 st
Edmonton Ab
T6G 2B7

Phone: (780) 407-8015
Fax: (780) 407-3009
Email: steven.joy <@t> capitalhealth.ca

This communication is intended for the sole use of the recipient to which it 
was addressed and may contain confidential, personal or privileged 
information. Please contact the sender immediately if you are not the 
intended recipient of this information and do not copy, distribute or take 
action relying on it. Any communication received in error, or subsequent 
reply, should be deleted or destroyed. Thank you.

_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

------------------------------

Message: 17
Date: Thu, 23 Apr 2009 19:47:17 -0500
From: "Joe Nocito" <jnocito <@t> satx.rr.com>
Subject: Re: [Histonet] Grossing
To: <schaundrawalton <@t> yahoo.com>, "Histonet"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <628FD19EBC8F444593AA7DAE461F282B <@t> JoePC>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
	reply-type=original

Schaundra,
we have residents and  2 PAs perform all the grossing. In real tight 
situations, we have a couple of qualified grossing histotechs we can rely on 
in a pinch. we have histotechs set up the specimens, but the residents and 
PA's gross alone. We are responsible for annotating any special procedures 
that need to be ordered. Our grossing cutoff time is 5:30 PM because of the 
start time of the tissue processors (we start embedding at 3:00 AM).

JTT
----- Original Message ----- 
From: "Schaundra Walton" <schaundrawalton <@t> yahoo.com>
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, April 23, 2009 1:25 PM
Subject: [Histonet] Grossing

We are evaluating some of our grossing procedures and cut off times and I 
was wondering what other people are doing.

Who does your grossing, pathologist, PA, or other qualified individuals?
Who does you accessioning, histotechs, support staff, or other?
What is your cut off time for grossing tissues?

Right now our pathologists gross all tissues, the histotechs accession, and 
grossing is done up until 6pm (which is when the processor begins).

Thanks for the info!

-Schaundra Walton HTL(ASCP)
Histology Supervisor
Swedish American Hospital
Rockford, IL

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------------------------------

Message: 18
Date: Thu, 23 Apr 2009 21:14:54 -0400
From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
Subject: RE: [Histonet] commercial control slides
To: "'Steven Joy'" <Steven.Joy <@t> capitalhealth.ca>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <779326963F2F4FF0964AFCF96B0452A4 <@t> HPPav2>
Content-Type: text/plain;	charset="us-ascii"

Frozen section of adrenal (from autopsy) is a great control. Freeze, cut
50-100 sections, put in a slide box, put in freezer, good for about a year.

Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Steven Joy
Sent: Thursday, April 23, 2009 2:39 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] commercial control slides

Can anyone recommend a brand or line of commercial control slides for oil
red O?

Thanks,
Steve Joy, BSc. MLT
Research and Development Technologist
5B2.03 Anatomical Pathology
University of Alberta Hospital
8440-112 st
Edmonton Ab
T6G 2B7

Phone: (780) 407-8015
Fax: (780) 407-3009
Email: steven.joy <@t> capitalhealth.ca

This communication is intended for the sole use of the recipient to which it
was addressed and may contain confidential, personal or privileged
information. Please contact the sender immediately if you are not the
intended recipient of this information and do not copy, distribute or take
action relying on it. Any communication received in error, or subsequent
reply, should be deleted or destroyed. Thank you.

_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 19
Date: Fri, 24 Apr 2009 14:58:08 +1000 (EST)
From: "Sarah Tarran" <sarah_tarran <@t> wmi.usyd.edu.au>
Subject: [Histonet] non-specific fluorescence in artery
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<2183.172.19.58.90.1240549088.squirrel <@t> www.wmi.usyd.edu.au>
Content-Type: text/plain;charset=iso-8859-1

Hi Histonetters,

We have a problem. We have optimised LSP-1 (leukocyte specific protein) on
spleen that has been fixed in 4% PFA/PBS for 2h, incubated in
18%sucrose/PBS overnight and then embedded in OCT. We now want to test it
on our arteries that have been fixed and frozen the same way as the
spleen. The staining protocol used is also identical for both tissues (see
below). However, unlike the specific staining we observe in spleen (on the
leukocytes), we observe very obvious non-specific background in the
arteries. Pretty much all of the stroma surrounding the tissue is
positively staining. The negative controls look good. Could anyone please
offer any reasons for what we're seeing? Our method is:

H2O to get rid of OCT
Citrate buffer - 10min at 60C (H2O bath)
Wash in running water - 10 min
Soaked 0.3%Triton/PBS - 3changes, 3 min each
Dako Protein block - 10 min
Primary Ab (rabbit anti-mouse), 1:50 diln - 1h
TBS with tween - 3x 3 changes
secondary Ab (Alexa Fluor 594, goat anti-rabbit), 1:800 - 1h
TBS with tween - 3x 3 changes
DAPI

Thanks,
Sarah

Sarah Tarran
Postdoctoral Fellow
Vascular Biology Research Centre,
Department of Surgery
Westmead Hospital, Westmead, NSW, 2145
sarah_tarran <@t> wmi.usyd.edu.au

------------------------------

Message: 20
Date: Fri, 24 Apr 2009 18:25:20 +0800
From: "TF" <tifei <@t> foxmail.com>
Subject: [Histonet] Doublecortin staining with Abcam antibody
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <200904241825152967272 <@t> foxmail.com>
Content-Type: text/plain;	charset="us-ascii"

hi all, I am staining 40 um brain frozen sections with Abcam anti-DCX (rabbit source).

With 10% goat serum blocking for 1 hour before primary antibody, I observed a lot of non-specific fluorescence on my slide. Anyone experienced this before?

Second, I did not perform the antigen retrieval before the staining. The sections are made on a microtome, and floating sections are then mounted on slides. 
But I added 0.3% triton to the primary antibody dilution. Will very long incubation (1-2 nights room temperature) with high concentration of triton affect the staining result? Such as "leaking" pattern of staining.

2009-04-24 

TF 

------------------------------

Message: 21
Date: Fri, 24 Apr 2009 10:23:14 -0400
From: Alyssa Peterson <alyssa <@t> alliedsearchpartners.com>
Subject: [Histonet] Opening In Texas
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<bbc6db3a0904240723t6e9366f1l9d69553df0bc2f64 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Happy Friday Everyone,

I have an opening for a histotech in Corpus Christi, TX. The position is
full time/permanent, and full relocation is paid. Please let me know if you
or anyone you know would be interested in this position. Thank you!
**
*Position: Histology Technician*

Department:Histology

Specialty Area:Allied Health

Supervisor's Title:Histology Manager

Work Schedule:FT Early Morning* MONDAY-FRIDAY WITH WEEKEND ROTATION*
 ------------------------------
 Experience Required:

·        Approximately 6moths-1 year on-the-job experience necessary
Job Description:

·        Performs various histological procedures to prepare and process
tissue specimens for examination by pathologist.

·        Provides information for diagnosis and treatment by conducting
histological procedures following policies and procedures.
Education:

·        HT certification by Board of Registry of the American Society of
Clinical Pathologists.

*Benefits: *

·     1.      Highly competitive compensation package, compensation
continuously reviewed and updated. Relocation Assistance. Medical Benefits,
and much more!

-- 
Alyssa Peterson
Allied Search Partners
O: 770.621.2639 ext. 4
F: 770.621.2640

------------------------------

Message: 22
Date: Fri, 24 Apr 2009 15:45:38 +0100
From: "Ian Montgomery" <ian.montgomery <@t> bio.gla.ac.uk>
Subject: [Histonet] Mega cassettes
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <706A809EABE9491C8BB67361AF32FBBC <@t> IBLS.GLA.AC.UK>
Content-Type: text/plain;	charset="us-ascii"

            Looking for a supplier of mega cassettes in the UK. VWR are a
wee bit expensive. 

Ian.

Dr. Ian Montgomery,

Histotechnology,

I.B.L.S. Support Unit,

Thomson Building,

University of Glasgow,

Glasgow,

G12 8QQ.

------------------------------

Message: 23
Date: Fri, 24 Apr 2009 10:33:50 -0500
From: "Montina Van Meter" <Montina.VanMeter <@t> pbrc.edu>
Subject: RE: [Histonet] Doublecortin staining with Abcam antibody
To: <tifei <@t> foxmail.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4FE7FB862E90E448AE32388E759220E5DC1757 <@t> pbrcas31.pbrc.edu>
Content-Type: text/plain;	charset="iso-8859-1"

Tifei,
   I routinely section rat brain at 40 microns and free-float them through the immuno protocol.  Here are a few 
   suggestions:

1. Did you perform a dilution series to optimize the best antibody concentration?

2. I antigen retrieve difficult antibodies (especially with thick sections) in a Decloaker (Biocare), thus opening up 
    the binding sites.

3. I'm assuming the tissue is fixed - we incubate the sections in a 1% sodium borohydride solution (after 
    Decloaker step) to rid excess aldehyde in tissue prior to the blocking step (20-30 mins.) Follow with PBS or 
    TBS rinses.

3.  We also use 0.3% triton - this is not a high concentration for 40 um sections.

4.  I use 0.3% triton in my antibody diluent  - overnight incubation.

5.  Make sure you have at least 3 rinses in between incubations to rid nonspecific "glow garbage" from the 
     secondary antibody.

Hope this helps,

Tina

Montina J. Van Meter
Lab Manager
Pennington Biomedical Research Center
6400 Perkins Rd.
Baton Rouge, LA  70808
225-763-2564

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of TF
Sent: Fri 4/24/2009 5:25 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Doublecortin staining with Abcam antibody

hi all, I am staining 40 um brain frozen sections with Abcam anti-DCX (rabbit source).

With 10% goat serum blocking for 1 hour before primary antibody, I observed a lot of non-specific fluorescence on my slide. Anyone experienced this before?

Second, I did not perform the antigen retrieval before the staining. The sections are made on a microtome, and floating sections are then mounted on slides.
But I added 0.3% triton to the primary antibody dilution. Will very long incubation (1-2 nights room temperature) with high concentration of triton affect the staining result? Such as "leaking" pattern of staining.

2009-04-24

TF
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Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 24
Date: Fri, 24 Apr 2009 11:39:39 -0400
From: "Amy Porter" <portera <@t> msu.edu>
Subject: Re: [Histonet] Mega cassettes
To: <ian.montgomery <@t> bio.gla.ac.uk>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <907F27D1D1AA41C9AF86E0AD4373C4F6 <@t> histolab>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
	reply-type=original

You could try Surgipath Medical. 

Amy S. Porter, HT (ASCP) QIHC
Investigative HistoPathology Laboratory - Supervisor
2201 Biomedical Physical Sciences Bldg.  Rm #2133
East Lansing, MI  48824-3320
Phone:  (517) 884-5026
Fax:  (517) 432-1368
Email:  portera <@t> msu.edu
Web:  www.humanpathology.msu.edu
----- Original Message ----- 
From: "Ian Montgomery" <ian.montgomery <@t> bio.gla.ac.uk>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, April 24, 2009 10:45 AM
Subject: [Histonet] Mega cassettes

>            Looking for a supplier of mega cassettes in the UK. VWR are a
> wee bit expensive. 
> 
> Ian.
> 
> 
> 
> 
> 
> Dr. Ian Montgomery,
> 
> Histotechnology,
> 
> I.B.L.S. Support Unit,
> 
> Thomson Building,
> 
> University of Glasgow,
> 
> Glasgow,
> 
> G12 8QQ.
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

------------------------------

Message: 25
Date: Thu, 23 Apr 2009 17:43:09 -0700
From: "Ryan McAdams" <mcadams <@t> u.washington.edu>
Subject: [Histonet] paraffin embedding
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<CBC95690762B46CEBB8475897533E55E <@t> pedom.peds.washington.edu>
Content-Type: text/plain;	charset="us-ascii"

I am trying to embed adult rat aortas in paraffin for histology purposes.
Do you have any recommendations on an optimal method to keep the aortas in a
vertical position while the paraffin solidifies?  I am hoping to do
transverse sectioning of the thoracic aortas to analyze wall thickness. 

Thanks,

Ryan

Ryan McAdams

Assistant Professor of Pediatrics 

Division of Neonatology University of Washington

Box 356320 Seattle, WA 98195-6320

Telephone: (206)-616-8246

------------------------------

Message: 26
Date: Fri, 24 Apr 2009 11:53:23 -0500
From: "Maruska, Ann" <AMaruska <@t> mngastro.com>
Subject: [Histonet] benzene/paraffin
To: "Histonet" <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<F7FD1B85F3C7784C97ECCBA98E2144C3019AA1FA <@t> mngi-ex01.mngastro.com>
Content-Type: text/plain;	charset="us-ascii"

Hi Histonetters,

In reviewing the MSDS sheets in our histology lab, I noticed that the
GemCut paraffin we are using contains 2% benzene.  I am concerned about
this.  Is this the exception or the norm?  Does other paraffin contain
products that would be considered hazardous or are they labeled in such
a manner that the consumer is unaware?

If someone is using a product that they know is safe and performs with
high quality, please let me know.

Thanks!

Ann

Ann Maruska

Pathology Lab manager

Minnesota Gastroenterology

email: amaruska <@t> mngastro.com

office: 651-605-3070

cell: 612-462-9699

fax: 612-870-5863

------------------------------

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End of Histonet Digest, Vol 65, Issue 42
****************************************