[Histonet] prostate bx processing time and staining time

Putnam, Jodi putnamj <@t> ggclinic.com
Sun Apr 19 11:11:33 CDT 2009

I was wondering if some of you would share your prostate bx processing times and staining times with me. 
I am using Richard Allan paraffin type 6. I'm cutting at 4um. Initially the pathologist said that they looked good but a fellow pathologist said that they could be too thick or over stained. I am using the same staining program that I use for all my other derm specimens. Do any of you use a certain program (time) or staining program for prostates? I can adjust the times on the processor to a shorter run or adjust the staining times in H & E but didn't want to just start trouble shooting without some feedback. I am worried that the overnight protocol on the processor might be too much for the bx's. At some labs we used a short run at others we used the over night run but there were other factors like the difference in paraffin etc.
Jodi Putnam (HT,ASCP)
Graves Gilbert Clinic
Pathology Department
201 Park Street
Bowling Green, KY 42102
(270) 393-2728 (voicemail)
(270) 393-2795 
Fax : (270) 393-2736
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