[Histonet] Acetone fixed tissue for PPFE

[Rene J Buesa]

Acetone acts as a dehydrating agent, so you do not need to go to ethanol now, but you will have to go to the ante medium (clearing agent) you use before the paraffin infiltration.
It is possible that because the tissue were in acetone for a more than "conventional" period of time, the tissues have become brittle and you may have problems when sectioning.
René J.

--- On Thu, 4/16/09, Bernice Frederick <b-frederick <@t> northwestern.edu> wrote:

From: Bernice Frederick <b-frederick <@t> northwestern.edu>
Subject: [Histonet] Acetone fixed tissue for PPFE
To: histonet <@t> lists.utsouthwestern.edu
Date: Thursday, April 16, 2009, 11:56 AM

Question:

I have acetone fixed tissue (someone trying to duplicate a paper) for
paraffin embedding? Should I go straight to absolute for processing?

Thanks,

Bernice

 

 

Bernice Frederick HTL (ASCP)

Northwestern University

Pathology Core Facility

ECOGPCO-RL 

710 N Fairbanks Court

Olson 8-421

Chicago,IL 60611

312-503-3723

 

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