[Histonet] Fli-1 antibody - Please help

[Young Kwun]

Dear Histonetters,

I was wondering whether anyone had success in Fli-1 immunohistochemistry on
formalin-fixed, paraffin embedded human sections.

I tried two polyclonal, commercial antibodies from abcam (ab15289) and Santa
Cruz Biotech's Fli-1 (C-19, sc-356) without any positive stainings.

Both antibodies were recommended for application on paraffin sections.
Abcam's application notes even mentioned that perform enzymatic antigen
retrieval BEFORE commencing with HIER procedure. The procedure still did not
give any stains and the morphology was unacceptable. I tried with/without
HIER with different buffers as well.

I used primary dilutions 1:50 - 1:200 as recommended and I am using Leica's
BondMax Autostainer. 

Any comments would be appreciated.

 

Young

 

 

 

Young Kwun

Senior Hospital Scientist

Dept. of Anatomical Pathology

Concord Hospital 

Concord NSW 2139 Australia

Tel)61-2-9767-6075

Fax)61-2-9767-8427

kwuny <@t> email.cs.nsw.gov.au