[Histonet] F4/80

[Anne Undersander]

Hi Histonet,

 

We have a working IHC protocol for F4/80 on mouse tissues.  However, when we
try to switch this procedure to IF it doesn't work.  Would anyone please
have any suggestions as to why this won't work?  The pretreatment that we
use is trypsin at 37 degrees for 15 min.  Does this enzyme inhibit the Cy5
which is the secondary fluorophore that we're using?  Any advice/help is
greatly appreciated!

 

Thanks!

Anne