[Histonet] Whole human feet

[Yak-Nam Wang]

Thanks Rene,

I have heard of the sliding microtomes and a colleague thought that as long
as I could fix and decalcify, it might be possible with one of these. I fear
I may have to pre-cut the foot in to manageable portions before processing.
Yes, if I find out how to do it I will definitely let you know!

Yak-Nam

On Fri, Apr 10, 2009 at 1:34 PM, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:

> As far as I know such a large thin section that also involves decalcifying
> hard bones has never been published. In the past E. Leitz (now Leica)
> manufactured sliding microtomes for large specimens but were used mostly to
> prepare thin brain sections embedded in celloidin but not for what you would
> like to do.
> If you are able to do it, for sure we all would like to know.
> René J.
>
> --- On *Fri, 4/10/09, Yak-Nam Wang <ynwang <@t> u.washington.edu>* wrote:
>
> From: Yak-Nam Wang <ynwang <@t> u.washington.edu>
> Subject: Re: [Histonet] Whole human feet
> To: "Woodward, Denise" <denise.woodward <@t> uconn.edu>
> Cc: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu
> >
> Date: Friday, April 10, 2009, 4:26 PM
>
>
> Denise,
>
> Thanks, someone else pointed out these images and they are indeed in the
> correct plane (http://www.nlm.nih.gov/research/visible/image/feet.jpg).  I
> think these are 1 mm sections from a frozen body, unstained. We were
> actually hoping that we could process feet (procured from a company),
> section and stain the sections with the basic H&E, modified Hart's,
> Picro
> sirius red and such like.
>
> Any help would be greatly appreciated.
> Thanks
> Yak-Nam
>
> On Fri, Apr 10, 2009 at 1:11 PM, Woodward, Denise <denise.woodward <@t> uconn.edu
> > wrote:
>
> > I believe there is a computerized image file of  an entire male and a
> > female human body somewhere on the WWW.  Maybe NIH??  These were whole
> mount
> > histologic sections. Don't know if the plane is correct for your needs
> with
> > regard to the sections of feet. News about it came out about 4-5 years
> ago.
> >
> >
> > -----Original Message-----
> > From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> > histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bernie Taupin
> > Sent: Wednesday, April 08, 2009 12:07 AM
> > To: Yak-Nam Wang; histonet <@t> lists.utsouthwestern.edu
> > Subject: Re: [Histonet] Whole human feet
> >
> > No offense, dude, but GROSS.
> >
> > That's probably why nobody has bitten yet, in regards to this query.
> >
> >
> >
> >
> > ________________________________
> > From: Yak-Nam Wang <ynwang <@t> u.washington.edu>
> > To: histonet <@t> lists.utsouthwestern.edu
> > Sent: Monday, April 6, 2009 8:04:54 PM
> > Subject: [Histonet] Whole human feet
> >
> > Hello all,
> >
> > If possible, we would like to obtain histological sections of adult human
> > feet (plane of the anterior-posterior surface). Does anyone know of
> > labs/groups that have done this or something similar?
> >
> > Thank you for your help
> > Yak-Nam Wang
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> >
> >
> >
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