[Histonet] Re: Recycled Formalin
Miyamoto, Garret T Mr CIV USA USAMEDCOM
garret.t.miyamoto <@t> us.army.mil
Wed Apr 8 14:17:17 CDT 2009
----- Original Message -----
From: histonet-request <@t> lists.utsouthwestern.edu
Date: Tuesday, April 7, 2009 7:15 pm
Subject: Histonet Digest, Vol 65, Issue 17
To: histonet <@t> lists.utsouthwestern.edu
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> Today's Topics:
>
> 1. Re: Large coverslips? (Va Paula Sicurello)
> 2. HI Downdraft fume extractor for sale (Cathy Mayton)
> 3. breaking glass jar/vials for MMA embedded specimens (Cathy
> Mayton) 4. AW: [Histonet] MAMMOGLOBIN (Gudrun Lang)
> 5. Re: AE1/AE3 nuclear staining (Rene J Buesa)
> 6. Re: AE1/AE3 nuclear staining (Mark Tarango)
> 7. RE: Tissue Capture Pen (Atoska Gentry)
> 8. RE: I'm outta here (Smith, Allen)
> 9. Controls needed! (Knutson, Deanne)
> 10. Fixation question - Cerebellar granular cells (Guillermo
> Palchik) 11. Recycled formalin (Richard Cartun)
> 12. RE: Unsubscribe (Tony Henwood)
> 13. RE: gi microwave processing (Tony Henwood)
> 14. RE: AE1/AE3 nuclear staining (Tony Henwood)
> 15. Re: Recycled formalin (Greg Dobbin)
> 16. Re: Fixation question - Cerebellar granular cells (TF)
> 17. Re: Staying... (Bernie Taupin)
> 18. Re: Staying... (Bernie Taupin)
> 19. RE: Unsubscribe (aa aa)
> 20. Re: Whole human feet (Bernie Taupin)
> 21. Re: "FREEZY" spray (Bernie Taupin)
> 22. Re: "FREEZY" spray (Akemi Allison-Tacha)
> 23. Re: "FREEZY" spray (Bernie Taupin)
>
>
> -------------------------------------------------------------------
> ---
>
> Message: 1
> Date: Tue, 7 Apr 2009 10:17:43 -0700 (PDT)
> From: Va Paula Sicurello <
> Subject: Re: [Histonet] Large coverslips?
> To: histonet <@t> lists.utsouthwestern.edu, yvan lindekens
> <
> Message-ID: <
> Content-Type: text/plain; charset=utf-8
>
>
> Hi Yvan,
>
> Does it have to be a coverslip? There are some mounting media that harden and form a barrier with optical qualities similar to that of glass.
>
> It might not be a perfect solution, but it might work.
>
> I think the stuff I used was called Crystal Mount (?).
>
> Paula :-)
>
> Paula Sicurello
> VA Medical Center San Diego
> Veterans Medical Research Foundation (VMRF)
> Core Research Imaging Center
> 3350 La Jolla Village Dr.., MC151
> San Diego, CA 92161
> 858-552-8585 x2397
>
>
> --- On Tue, 4/7/09, yvan lindekens < wrote:
>
> > From: yvan lindekens <
> > Subject: [Histonet] Large coverslips?
> > To: histonet <@t> lists.utsouthwestern.edu
> > Date: Tuesday, April 7, 2009, 9:05 AM
> >
> > Hi all,
> >
> > I���m looking for some kind of a DIY coverslip or a tape,
> > plastic foil��� anything usable to cover some large slides
> > (+/- 4.5cm * 15cm = 1.8inch * 5.9inch) containing botanical
> > and zoological sections mounted in Canada Balsam.
> >
> > Does anyone has a (cheap���) solution for this? It
> > doesn���t need to be pristine optical quality as the slides
> > are primarely intended to be used on an overhead projector
> > in the class room, but the possibility of viewing them under
> > low power (40x - 100x) would be a real advantage.
> >
> > Thanks in advance four your wisdom and knowledge!
> >
> > Yvan.
> >
> >
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>
>
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Tue, 7 Apr 2009 10:23:02 -0700
> From: "Cathy Mayton" <
> Subject: [Histonet] HI Downdraft fume extractor for sale
> To: <
> Message-ID: <
> Content-Type: text/plain; charset="iso-8859-1"
>
> I have a HI downdraft fume extractor for $50 and we will provide carbon to refill the drawer.
>
> Cathy A. Mayton
> Wasatch Histo Consultants, Inc.
> 775-625-4425
>
>
>
> ------------------------------
>
> Message: 3
> Date: Tue, 7 Apr 2009 10:28:44 -0700
> From: "Cathy Mayton" <
> Subject: [Histonet] breaking glass jar/vials for MMA embedded
> specimens
> To: <
> Message-ID: <
> Content-Type: text/plain; charset="iso-8859-1"
>
> Yes, the scintillation vials are notorious for shattering in the freezer. However, if you put them in a plastic container with a lid on, the shattered glass will be contained and not all over your freezer. When breaking larger glass containers, again place them in the freezer, remove from the freezer after 20 minutes or so, take the lid off, wrap jar in paper towels and hold the ends closed. Wear glovesand eye protection in case shards of glass escape. Strike the jar with a hammer and unroll the paper towel over a garbage can. Rinse the block in tap water to remove any small shards of glass. This is a great way to vent frustration!!
>
> Cathy A. Mayton
> Wasatch Histo Consultants, Inc.
>
>
>
> ------------------------------
>
> Message: 4
> Date: Tue, 7 Apr 2009 19:36:14 +0200
> From: "Gudrun Lang" <
> Subject: AW: [Histonet] MAMMOGLOBIN
> To: "'Vickroy, Jim'" <
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <
> Content-Type: text/plain; charset="iso-8859-1"
>
> I tried also this antibody on Ventana Benchmark. Pity, I had no results.
> Gudrun
>
> -----Urspr�ngliche Nachricht-----
> Von: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Vickroy,
> Jim
> Gesendet: Dienstag, 07. April 2009 17:54
> An: 'Histonet <@t> lists.utsouthwestern.edu'
> Betreff: [Histonet] MAMMOGLOBIN
>
>
> Has anyone used the DAKO predilute antibody, Mammoglobin, on the Ventana
> Benchmark XT? And if so.....could you share the protocol you used?
> Finally looking on the Dako website it appears that they have ready to use,
> could you share with me which antibody should be used with the Ventana
> I-view detection kit?
>
> Thanks
>
>
>
>
> Jim Vickroy BS, HT(ASCP)
> Technical Supervisor - Surgical and Autopsy Pathology
> Memorial Medical Center
> 217-788-4046
> vickroy.jim <@t> mhsil.com
>
>
>
> This message (including any attachments) contains confidential information
> intended for a specific individual and purpose, and is protected by law. If
> you are not the intended recipient, you should delete this message. Any
> disclosure, copying, or distribution of this message, or the taking of any
> action based on it, is strictly prohibited.
>
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>
>
>
>
> ------------------------------
>
> Message: 5
> Date: Tue, 7 Apr 2009 12:16:44 -0700 (PDT)
> From: Rene J Buesa <
> Subject: Re: [Histonet] AE1/AE3 nuclear staining
> To: histonet <@t> lists.utsouthwestern.edu, Tammy Barnhart
> <
> Message-ID: <
> Content-Type: text/plain; charset=iso-8859-1
>
> Have you changed any of the reagents in your X-press tissue processor or other aspect of your tissue processing protocol? This could be�a factor.
> Ren� J.
>
> --- On Tue, 4/7/09, Tammy Barnhart < wrote:
>
> From: Tammy Barnhart <
> Subject: [Histonet] AE1/AE3 nuclear staining
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Tuesday, April 7, 2009, 12:53 PM
>
> We have recently been having dark nuclear staining with our AE1/AE3
> antibody. This is a recent event and we cannot figure out what is going
> on. It started gradually and has been increasing in intensity. We have
> not changed antibody lots or any other part of our protocol. Has anyone
> seen this before? Any suggestions on what is happening here?
>
> Tammy Barnhart, BS, HTL(ASCP)
>
> Avera McKennan Hospital
>
>
>
> -----------------------------------------
> Confidentiality Notice: This e-mail message, including any attachments, is for
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> contact the sender by reply e-mail and destroy all copies of the original
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 6
> Date: Tue, 7 Apr 2009 12:22:13 -0700
> From: Mark Tarango <
> Subject: Re: [Histonet] AE1/AE3 nuclear staining
> To: Tammy Barnhart <
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <
> Content-Type: text/plain; charset=ISO-8859-1
>
> When was the last time you checked the pH of your retrieval solution? Or
> are you using an enzyme?
>
> Mark Tarango
>
> On Tue, Apr 7, 2009 at 9:53 AM, Tammy Barnhart
> <wrote:
>
> > We have recently been having dark nuclear staining with our AE1/AE3
> > antibody. This is a recent event and we cannot figure out what is going
> > on. It started gradually and has been increasing in intensity. We have
> > not changed antibody lots or any other part of our protocol. Has anyone
> > seen this before? Any suggestions on what is happening here?
> >
> > Tammy Barnhart, BS, HTL(ASCP)
> >
> > Avera McKennan Hospital
> >
> >
> >
> > -----------------------------------------
> > Confidentiality Notice: This e-mail message, including any attachments, is
> > for the sole use of the intended recipient(s) and may contain confidential
> > and privileged information. Any unauthorized review, use, disclosure, or
> > distribution is prohibited. If you are not the intended recipient, please
> > contact the sender by reply e-mail and destroy all copies of the original
> > message.
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>
> ------------------------------
>
> Message: 7
> Date: Tue, 07 Apr 2009 14:28:16 -0500
> From: Atoska Gentry <
> Subject: [Histonet] RE: Tissue Capture Pen
> To: Histonet <
> Message-ID: <
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>
> hello, if any of you are using and/or have used the Ted Pella, Inc.
> Tissue Capture Pen will you be so kind as to share with me your
> experience (s), pros, cons and whatever else you deem necessary? Also,
> does it's use require special glass slides? Any info you can provide on
> it's use ASAP will be much appreciated. Thank you kindly, Atoska
>
>
>
> ------------------------------
>
> Message: 8
> Date: Tue, 7 Apr 2009 15:40:35 -0400
> From: "Smith, Allen" <
> Subject: [Histonet] RE: I'm outta here
> To: "'Histonet <@t> lists.utsouthwestern.edu'"
> <
> Message-ID:
> <
>
> Content-Type: text/plain; charset="us-ascii"
>
> I can spot spam and o.t. posts and delete them in a half second each. The questions on Histonet make me aware of the extent of use of special stains, and the potential for new stains. Jjob postings are forwarded to the career service office here. The safety warnings are often very useful. HIstonet is a great source of information on antigen retrieval techniques and antibodies. I would really miss Histonet.
> --Allen A Smith, Ph.D.
> Barry University School of Podiatric Medicine
>
>
> ------------------------------
>
> Message: 9
> Date: Tue, 7 Apr 2009 15:06:13 -0500
> From: "Knutson, Deanne" <
> Subject: [Histonet] Controls needed!
> To: "'histonet <@t> lists.utsouthwestern.edu'"
> <
> Message-ID: <
> Content-Type: text/plain
>
> We are looking for H. Pylori control tissue and also GMS/fungus control
> tissue. Is there anyone out there that might have extra to share? We have
> good GRAM control blocks that we would be happy to exchange. Please let me
> know if you can help us out. Thank you!
>
>
>
> Deanne Knutson
>
> Anatomic Pathology Supervisor
>
> St. Alexius Medical Center
>
> Bismarck, N. Dak. 58506
>
> 701-530-6730
>
> Fax 701-530-6735
>
>
>
> ------------------------------
>
> Message: 10
> Date: Tue, 7 Apr 2009 16:59:58 -0400
> From: Guillermo Palchik <
> Subject: [Histonet] Fixation question - Cerebellar granular cells
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <
> Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes
>
> Dear Histoneters,
>
> I am doing some IHC on rat cerebellar granular cells (NOT TISSUE) and
> I need to fix them with PF.
> I have gotten 4 different answers on how to go about this, and I
> wanted to run this by the list to see what you think.
>
> 1) Fix in COLD, 4% PF in 1x PBS
>
> 2) Fix in COLD, 4% PF in 1x PBS with 4% Sucrose.
>
> 3) Fix in WARM (37 C) ,in 4% PF in 1x PBS.
>
> 4) Fix in WARM (37 C), in 1x PBS with 4% Sucrose.
>
> I get the fact that the PF might need to be at 37 C, since it is the
> temperature that the cells are in the incubator and it would probably
> temperature shock them. What about the sucrose? does it remove the
> water?
>
> I'd appreciate your thoughts...
> Best,
>
> Guillermo
>
> Guillermo Palchik
> gp62 <@t> georgetown.edu
>
>
>
>
>
>
>
>
>
> ------------------------------
> Richard,
We do use recycled formalin for our surgical and autopsy tissues. We have a "Procycler" from B/R Instrument Corporation that recycles used formalin. It comes with a kit to test the recycled product and also a "buffering solution" to add to it. We have no problems with fixation using recycled formalin.
Garret Miyamoto
Tripler Army Medical Center
> Message: 11
> Date: Tue, 07 Apr 2009 18:30:04 -0400
> From: "Richard Cartun" <
> Subject: [Histonet] Recycled formalin
> To: "Histonet" <
> Message-ID: <
> Content-Type: text/plain; charset=US-ASCII
>
> Is anyone using recycled formalin for primary fixation of either surgical or autopsy tissue? Thanks.
>
> Richard
>
> Richard W. Cartun, Ph.D.
> Director, Histology & Immunopathology
> Director, Biospecimens
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 545-1596
> (860) 545-0174 Fax
>
>
>
>
> ------------------------------
>
> Message: 12
> Date: Wed, 8 Apr 2009 08:44:12 +1000
> From: "Tony Henwood" <
> Subject: RE: [Histonet] Unsubscribe
> To: <, <
> Message-ID: <
> Content-Type: text/plain; charset="us-ascii"
>
> Sophie,
> I for one would never throw a torrent of abuse at any one (mmm unless
> they are politicians - in between football seasons!)
>
> So if my (and most others on Histonet) comments are not of some worth
> then we apologise.
>
> We need to try harder.
>
> (also please remember the delete key, I unfortunately have to regularly
> use it)
>
> Regards
>
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
> Laboratory Manager & Senior Scientist
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> S.J.Ainsworth <@t> bsms.ac.uk
> Sent: Tuesday, 7 April 2009 5:53 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Unsubscribe
>
>
> Please can you unsubscribe me from this mailing list. As an
> inexperienced histologist trying to find my feet I no longer feel like I
> could ask any questions as I'm sure I would get a torrent of abuse back
> instead of any helpful comments.
>
>
>
> Sophie Ainsworth
> Brighton and Sussex Medical School
> Medical Research Building
> Falmer
> East Sussex
> BN1 9PX
>
> Tel: #44 1273 877886
> Fax: #44 1273 877884
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> *********************************************************************
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>
> Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead
>
> This note also confirms that this email message has been
> virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.
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>
>
>
>
> ------------------------------
>
> Message: 13
> Date: Wed, 8 Apr 2009 08:48:53 +1000
> From: "Tony Henwood" <
> Subject: RE: [Histonet] gi microwave processing
> To: <, <
> Message-ID: <
> Content-Type: text/plain; charset="us-ascii"
>
> Nancy,
>
> Best advice I can offer is to ensure as much fixation as you can.
> We start with 30 minutes at 30oC and the ramp it up to 40oC for 30-60
> minutes, rinse in 70% ethanol (5min)then continue microwave processing
> with isopropanol and wax. You may also consider decreasing the
> processing temperatures.
>
> GI biopsies being quite small do not need over-the-top processing, but
> in my experience, fixation is still the key.
>
> Regards
>
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
> Laboratory Manager & Senior Scientist
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> MAGODLEY <@t> aol.com
> Sent: Tuesday, 7 April 2009 10:11 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] gi microwave processing
>
>
> I am starting a gi lab and using a microwave processor for my first
> time.
> Any suggestions? SHUR Wave processor. Thanks, Nancy
> **************A Good Credit Score is 700 or Above. See yours in just 2
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> *********************************************************************
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>
> Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead
>
> This note also confirms that this email message has been
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>
>
> ------------------------------
>
> Message: 14
> Date: Wed, 8 Apr 2009 10:06:52 +1000
> From: "Tony Henwood" <
> Subject: RE: [Histonet] AE1/AE3 nuclear staining
> To: "Tammy Barnhart" <,
> <
> Message-ID: <
> Content-Type: text/plain; charset="us-ascii"
>
> Possibly the concentration of hydrogen peroxide in the DAB solution has
> been slowly increasing (maybe micropipette creep?)
>
> Regards
>
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
> Laboratory Manager & Senior Scientist
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tammy
> Barnhart
> Sent: Wednesday, 8 April 2009 2:54 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] AE1/AE3 nuclear staining
>
>
> We have recently been having dark nuclear staining with our AE1/AE3
> antibody. This is a recent event and we cannot figure out what is going
> on. It started gradually and has been increasing in intensity. We have
> not changed antibody lots or any other part of our protocol. Has anyone
> seen this before? Any suggestions on what is happening here?
>
> Tammy Barnhart, BS, HTL(ASCP)
>
> Avera McKennan Hospital
>
>
>
> -----------------------------------------
> Confidentiality Notice: This e-mail message, including any attachments,
> is for the sole use of the intended recipient(s) and may contain
> confidential and privileged information. Any unauthorized review, use,
> disclosure, or distribution is prohibited. If you are not the intended
> recipient, please contact the sender by reply e-mail and destroy all
> copies of the original message.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> *********************************************************************
> This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.
>
> Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead
>
> This note also confirms that this email message has been
> virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.
> **********************************************************************
>
>
>
>
> ------------------------------
>
> Message: 15
> Date: Tue, 07 Apr 2009 21:55:26 -0300
> From: "Greg Dobbin" <
> Subject: Re: [Histonet] Recycled formalin
> To: <,<
> Message-ID: <
> Content-Type: text/plain; charset=US-ASCII
>
> Yes. ??
> Greg
>
> Greg Dobbin, R.T.
> Chief Technologist, Anatomic Pathology
> Dept. of Laboratory Medicine,
> Queen Elizabeth Hospital,
> P.O. Box 6600
> Charlottetown, PE C1A 8T5
> Phone: (902) 894-2337
> Fax: (902) 894-2385
>
> "I find that the harder I work, the
> more luck I seem to have."
> - Thomas Jefferson
>
> >>> "Richard Cartun" < 04/07/09 7:30 PM >>>
> Is anyone using recycled formalin for primary fixation of either
> surgical or autopsy tissue? Thanks.
>
> Richard
>
> Richard W. Cartun, Ph.D.
> Director, Histology & Immunopathology
> Director, Biospecimens
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 545-1596
> (860) 545-0174 Fax
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> -------------------------
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> -------------------------
>
>
>
>
> ------------------------------
>
> Message: 16
> Date: Wed, 8 Apr 2009 11:07:21 +0800
> From: "TF" <
> Subject: Re: [Histonet] Fixation question - Cerebellar granular cells
> To: "Guillermo Palchik" <, "histonet"
> <
> Message-ID: <
> Content-Type: text/plain; charset="gb2312"
>
> Hi, i normally work on tissue.
> I think 4% PFA in 0.1M PB is fine. so approach 3.
>
>
> 2009-04-08
>
>
>
> TF
>
>
>
> �������� Guillermo Palchik
> ���������� 2009-04-08 05:03:13
> �������� histonet
> ������
> ������ [Histonet] Fixation question - Cerebellar granular cells
>
> Dear Histoneters,
> I am doing some IHC on rat cerebellar granular cells (NOT TISSUE) and
> I need to fix them with PF.
> I have gotten 4 different answers on how to go about this, and I
> wanted to run this by the list to see what you think.
> 1) Fix in COLD, 4% PF in 1x PBS
> 2) Fix in COLD, 4% PF in 1x PBS with 4% Sucrose.
> 3) Fix in WARM (37 C) ,in 4% PF in 1x PBS.
> 4) Fix in WARM (37 C), in 1x PBS with 4% Sucrose.
> I get the fact that the PF might need to be at 37 C, since it is the
> temperature that the cells are in the incubator and it would probably
> temperature shock them. What about the sucrose? does it remove the
> water?
> I'd appreciate your thoughts...
> Best,
> Guillermo
> Guillermo Palchik
> gp62 <@t> georgetown.edu
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ------------------------------
>
> Message: 17
> Date: Tue, 7 Apr 2009 20:40:32 -0700 (PDT)
> From: Bernie Taupin <
> Subject: Re: [Histonet] Staying...
> To: "Bartlett, Jeanine \(CDC/CCID/NCZVED\)" <, Marla
> Thomas <, histonet <@t> lists.utsouthwestern.edu
> Message-ID: <
> Content-Type: text/plain; charset=us-ascii
>
> Yes, Well-done to all of you who have decided to stay! It really separates the wheat from the chaff, as it were. Or the weenies from the real adults, more or less. And even the French-speakers from the non-French-speakers, in the case of Kemlo/Rene. Ha ha. That was a joke. Learn to laugh a little, folks.
>
> I, myself, have decided to stay.
>
> I'll try to play more nicely. Here's hoping the rest of you can be so cool about it all.
>
> SO BIG UPS TO ALL THE HISTOLOGISTS IN THE HOUSE! CAN I GET A WHUT-WHUT!
>
>
>
>
> ________________________________
> From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" <
> To: Marla Thomas <; histonet <@t> lists.utsouthwestern.edu
> Sent: Tuesday, April 7, 2009 8:10:22 AM
> Subject: RE: [Histonet] Staying...
>
> Amen!
>
>
> Jeanine Bartlett
> Infectious Diseases Pathology Branch
> (404) 639-3590
> jeanine.bartlett <@t> cdc.hhs.gov
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Marla
> Thomas
> Sent: Tuesday, April 07, 2009 7:48 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Staying...
>
> I am staying on the list-serve. I have learned a lot from people on
> this list-serve. I have been in the field since 1970 and I too am still
> learning things. No one has all of the answers, and sometimes there
> isn't just one answer.
>
> I am staying on the list-serve, and if my input can help one person, or
> 15 different responses can help me, then the list-serve works. There
> are still people out there asking questions in between the bickering.
>
> Marla Thomas, HT(ASCP)
> Litton Pathology Associates, PC
> 700 NW Hunter Dr.
> Blue Springs, MO 64015
> Phone: 816-229-6449 Fax:816-874-4400
>
> CONFIDENTIALITY NOTICE
> This message and any included attachments are from Litton Pathology
> Associates, P.C. and are intended only for the addressee. The
> information contained in this message is confidential and may contain
> privileged, confidential, proprietary and/or exemption from disclosure
> under applicable law. Unauthorized forwarding, printing, copying,
> distribution, or use of such information is strictly prohibited and may
> be unlawful. If you are not the addressee, please promptly delete this
> message and notify the sender of the delivery error by e-mail or you may
> call 816-229-6449 and ask for the HIPAA/Compliance Coordinator.
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 18
> Date: Tue, 7 Apr 2009 20:40:32 -0700 (PDT)
> From: Bernie Taupin <
> Subject: Re: [Histonet] Staying...
> To: "Bartlett, Jeanine \(CDC/CCID/NCZVED\)" <, Marla
> Thomas <, histonet <@t> lists.utsouthwestern.edu
> Message-ID: <
> Content-Type: text/plain; charset=us-ascii
>
> Yes, Well-done to all of you who have decided to stay! It really separates the wheat from the chaff, as it were. Or the weenies from the real adults, more or less. And even the French-speakers from the non-French-speakers, in the case of Kemlo/Rene. Ha ha. That was a joke. Learn to laugh a little, folks.
>
> I, myself, have decided to stay.
>
> I'll try to play more nicely. Here's hoping the rest of you can be so cool about it all.
>
> SO BIG UPS TO ALL THE HISTOLOGISTS IN THE HOUSE! CAN I GET A WHUT-WHUT!
>
>
>
>
> ________________________________
> From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" <
> To: Marla Thomas <; histonet <@t> lists.utsouthwestern.edu
> Sent: Tuesday, April 7, 2009 8:10:22 AM
> Subject: RE: [Histonet] Staying...
>
> Amen!
>
>
> Jeanine Bartlett
> Infectious Diseases Pathology Branch
> (404) 639-3590
> jeanine.bartlett <@t> cdc.hhs.gov
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Marla
> Thomas
> Sent: Tuesday, April 07, 2009 7:48 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Staying...
>
> I am staying on the list-serve. I have learned a lot from people on
> this list-serve. I have been in the field since 1970 and I too am still
> learning things. No one has all of the answers, and sometimes there
> isn't just one answer.
>
> I am staying on the list-serve, and if my input can help one person, or
> 15 different responses can help me, then the list-serve works. There
> are still people out there asking questions in between the bickering.
>
> Marla Thomas, HT(ASCP)
> Litton Pathology Associates, PC
> 700 NW Hunter Dr.
> Blue Springs, MO 64015
> Phone: 816-229-6449 Fax:816-874-4400
>
> CONFIDENTIALITY NOTICE
> This message and any included attachments are from Litton Pathology
> Associates, P.C. and are intended only for the addressee. The
> information contained in this message is confidential and may contain
> privileged, confidential, proprietary and/or exemption from disclosure
> under applicable law. Unauthorized forwarding, printing, copying,
> distribution, or use of such information is strictly prohibited and may
> be unlawful. If you are not the addressee, please promptly delete this
> message and notify the sender of the delivery error by e-mail or you may
> call 816-229-6449 and ask for the HIPAA/Compliance Coordinator.
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 19
> Date: Tue, 7 Apr 2009 20:56:34 -0700 (PDT)
> From: aa aa <
> Subject: RE: [Histonet] Unsubscribe
> To: S.J.Ainsworth <@t> bsms.ac.uk, histonet <@t> lists.utsouthwestern.edu, Tony
> Henwood <
> Message-ID: <
> Content-Type: text/plain; charset=us-ascii
>
> Dear Histonetters:
>
>
> It sure is a real shame to see so many familiar names leave the list
> just due to a little annoyance. Sorry to send yet *another* note about
> all of this to the whole list, but I wanted to pass on a link my
> supervisor sent to me when we were having similar real-life crisis in
> my lab... it really does put things into perfect perspective:
> http://smouch.net/lol/
>
>
>
> Have a great day everyone, and keep HistoNetting!
>
>
>
> ~L.
>
>
>
> --- On Tue, 4/7/09, Tony Henwood < wrote:
> From: Tony Henwood <
> Subject: RE: [Histonet] Unsubscribe
> To: S.J.Ainsworth <@t> bsms.ac.uk, histonet <@t> lists.utsouthwestern.edu
> Date: Tuesday, April 7, 2009, 6:44 PM
>
> Sophie,
> I for one would never throw a torrent of abuse at any one (mmm unless
> they are politicians - in between football seasons!)
>
> So if my (and most others on Histonet) comments are not of some worth
> then we apologise.
>
> We need to try harder.
>
> (also please remember the delete key, I unfortunately have to regularly
> use it)
>
> Regards
>
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
> Laboratory Manager & Senior Scientist
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> S.J.Ainsworth <@t> bsms.ac.uk
> Sent: Tuesday, 7 April 2009 5:53 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Unsubscribe
>
>
> Please can you unsubscribe me from this mailing list. As an
> inexperienced histologist trying to find my feet I no longer feel like I
> could ask any questions as I'm sure I would get a torrent of abuse back
> instead of any helpful comments.
>
>
>
> Sophie Ainsworth
> Brighton and Sussex Medical School
> Medical Research Building
> Falmer
> East Sussex
> BN1 9PX
>
> Tel: #44 1273 877886
> Fax: #44 1273 877884
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> *********************************************************************
> This email and any files transmitted with it are confidential and intended
> solely for the use of the individual or entity to whom they are addressed. If
> you are not the intended recipient, please delete it and notify the sender.
>
> Views expressed in this message and any attachments are those of the individual
> sender, and are not necessarily the views of The Children's Hospital at
> Westmead
>
> This note also confirms that this email message has been
> virus scanned and although no computer viruses were detected, The Childrens
> Hospital at Westmead accepts no liability for any consequential damage resulting
> from email containing computer viruses.
> **********************************************************************
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 20
> Date: Tue, 7 Apr 2009 21:07:13 -0700 (PDT)
> From: Bernie Taupin <
> Subject: Re: [Histonet] Whole human feet
> To: Yak-Nam Wang <,
> histonet <@t> lists.utsouthwestern.edu
> Message-ID: <
> Content-Type: text/plain; charset=us-ascii
>
> No offense, dude, but GROSS.
>
> That's probably why nobody has bitten yet, in regards to this query.
>
>
>
>
> ________________________________
> From: Yak-Nam Wang <
> To: histonet <@t> lists.utsouthwestern.edu
> Sent: Monday, April 6, 2009 8:04:54 PM
> Subject: [Histonet] Whole human feet
>
> Hello all,
>
> If possible, we would like to obtain histological sections of adult human
> feet (plane of the anterior-posterior surface). Does anyone know of
> labs/groups that have done this or something similar?
>
> Thank you for your help
> Yak-Nam Wang
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 21
> Date: Tue, 7 Apr 2009 21:06:44 -0700 (PDT)
> From: Bernie Taupin <
> Subject: Re: [Histonet] "FREEZY" spray
> To: Jennifer MacDonald <, Ingles Claire
> <
> Cc: Histonet <@t> lists.utsouthwestern.edu,
> histonet-bounces <@t> lists.utsouthwestern.edu
> Message-ID: <
> Content-Type: text/plain; charset=us-ascii
>
> > Does this apply to Liquid Nitrogen as well? I'd hate to have to try to cut fatty sections in the cryostat without it!
>
> I do mostly cryo, and I've never used liquid nitrogen to chill anything within the cryostat. What's that all about?
>
> I find that it leaves to many ice crystal artifacts anyway. Not cold enough fast enough. You might be making it harder on yourself... just set the temperature of the cryostat for the appropriate temperature of whatever tissue youre cutting, and you should have no need for a crutch, whether it be fluoroethane or liquid nitrogen.
>
> kisses, flowers and rainbows,
> Bernie Taupin, King of Cryomicrotomy, Esq.
>
>
>
>
>
> ------------------------------
>
> Message: 22
> Date: Tue, 7 Apr 2009 22:03:04 -0700 (PDT)
> From: Akemi Allison-Tacha <
> Subject: Re: [Histonet] "FREEZY" spray
> To: Jennifer MacDonald <, Ingles Claire
> <, Bernie Taupin <
> Cc: Histonet <@t> lists.utsouthwestern.edu,
> histonet-bounces <@t> lists.utsouthwestern.edu
> Message-ID: <
> Content-Type: text/plain; charset=iso-8859-1
>
> Bernie,
> I used to use the method below for FS on muscles at Emanual Hospital in Portland OR. Since we had such wonderful success, we incorporated this method for all FS. �
> Years later, I developed the "MATSSE (pH3.4) 1-Step Trichrome Stain Kit" (Modified Trichrome Method for Muscle Biopsies Cut on Frozen Sections) for Biocare Medical. �This kit has long since discontinued by the way...This came from Biocare's Data Sheet.
>
>
> NOTE: Muscle tissue should be suitably obtained and
> frozen within 30 minutes after excision.� The usual
> method of freezing is to first mount a transverse section of the muscle on a
> chuck using 10% tragacanth gum or equivalent commercial frozen section mounting
> media as the adhesive.� The chuck
> with the mounted specimen is then immersed in prechilled isopentane until frozen.� Isopentane is precooled when a container of the substance is
> placed into liquid nitrogen.� At a
> temperature of -160� C, the isopentane has a slightly syrupy
> consistency.� Care should be
> taken to remove the muscle sample when freezing is complete, usually after 25
> to 30 seconds.� Too short a
> freezing time produces artifacts; prolonged freezing can produce cracking of
> the block.
>
> �
>
> NOTE:�If
> the sample cannot be sectioned immediately after freezing, it may be wrapped in
> foil and placed in a plastic-lidded container along with a small amount of ice
> for moisture.� Specimens may be
> stored at -70�
> C until sectioned.�
>
>
>
>
>
> Regards,Akemi
> Akemi Allison-Tacha BS, HT (ASCP) HTL
>
> Histology Manager
>
> Associated Pathology Medical Group Laboratories
>
> 105A Cooper Court, Los Gatos, CA 95032
>
> Cell: (425) 941-4287
>
> E-Mail: akemiat3377 <@t> yahoo.com
>
> --- On Tue, 4/7/09, Bernie Taupin < wrote:
>
> From: Bernie Taupin <
> Subject: Re: [Histonet] "FREEZY" spray
> To: "Jennifer MacDonald" <, "Ingles Claire" <
> Cc: Histonet <@t> lists.utsouthwestern.edu, histonet-bounces <@t> lists.utsouthwestern.edu
> Date: Tuesday, April 7, 2009, 9:06 PM
>
> > Does this apply to Liquid Nitrogen as well? I'd hate to have to try to cut fatty sections in the cryostat without it!
>
> I do mostly cryo, and I've never used liquid nitrogen to chill anything within the cryostat. What's that all about?
>
> I find that it leaves to many ice crystal artifacts anyway. Not cold enough fast enough. You might be making it harder on yourself... just set the temperature of the cryostat for the appropriate temperature of whatever tissue youre cutting, and you should have no need for a crutch, whether it be fluoroethane or liquid nitrogen.
>
> kisses, flowers and rainbows,
> Bernie Taupin, King of Cryomicrotomy, Esq.
>
>
>
> � � �
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ------------------------------
>
> Message: 23
> Date: Tue, 7 Apr 2009 22:11:53 -0700 (PDT)
> From: Bernie Taupin <
> Subject: Re: [Histonet] "FREEZY" spray
> To: Akemi Allison-Tacha <, Jennifer MacDonald
> <, Ingles Claire <
> Cc: Histonet <@t> lists.utsouthwestern.edu,
> histonet-bounces <@t> lists.utsouthwestern.edu
> Message-ID: <
> Content-Type: text/plain; charset=iso-8859-1
>
> I use isopentane suspended in liquid nitrogen, too.
>
> sorry if this sounds curt, but due to your cut-and-pasting, im not entirely sure what point youre trying to get at...?
>
>
>
>
> ________________________________
> From: Akemi Allison-Tacha <
> To: Jennifer MacDonald <; Ingles Claire <; Bernie Taupin <
> Cc: Histonet <@t> lists.utsouthwestern.edu; histonet-bounces <@t> lists.utsouthwestern.edu
> Sent: Wednesday, April 8, 2009 1:03:04 AM
> Subject: Re: [Histonet] "FREEZY" spray
>
>
> Bernie,
>
> I used to use the method below for FS on muscles at Emanual Hospital in Portland OR. Since we had such wonderful success, we incorporated this method for all FS.
>
> Years later, I developed the "MATSSE (pH3.4) 1-Step Trichrome Stain Kit" (Modified Trichrome Method for Muscle Biopsies Cut on Frozen Sections) for Biocare Medical. This kit has long since discontinued by the way....This came from Biocare's Data Sheet.
>
> NOTE:Muscle tissue should be suitably obtained and
> frozen within 30 minutes after excision. The usual
> method of freezing is to first mount a transverse section of the muscle on a
> chuck using 10% tragacanth gum or equivalent commercial frozen section mounting
> media as the adhesive. The chuck
> with the mounted specimen is then immersed in prechilled isopentane until frozen. Isopentane is precooled when a container of the substance is
> placed into liquid nitrogen. At a
> temperature of -160� C, the isopentane has a slightly syrupy
> consistency. Care should be
> taken to remove the muscle sample when freezing is complete, usually after 25
> to 30 seconds. Too short a
> freezing time produces artifacts; prolonged freezing can produce cracking of
> the block.
>
> NOTE: If
> the sample cannot be sectioned immediately after freezing, it may be wrapped in
> foil and placed in a plastic-lidded container along with a small amount of ice
> for moisture. Specimens may be
> stored at -70� C until sectioned.
> Regards,
> Akemi
>
> Akemi Allison-Tacha BS, HT (ASCP) HTL
> Histology Manager
> Associated Pathology Medical Group Laboratories
> 105A Cooper Court, Los Gatos, CA 95032
> Cell: (425) 941-4287
> E-Mail: akemiat3377 <@t> yahoo.com
>
> --- On Tue, 4/7/09, Bernie Taupin < wrote:
>
>
> From: Bernie Taupin <
> Subject: Re: [Histonet] "FREEZY" spray
> To: "Jennifer MacDonald" <, "Ingles Claire" <
> Cc: Histonet <@t> lists.utsouthwestern.edu, histonet-bounces <@t> lists.utsouthwestern.edu
> Date: Tuesday, April 7, 2009, 9:06 PM
>
>
> > Does this apply to Liquid Nitrogen as well? I'd hate to have to try to cut fatty sections in the cryostat without it!
>
> I do mostly cryo, and I've never used liquid nitrogen to chill anything within the cryostat. What's that all about?
>
> I find that it leaves to many ice crystal artifacts anyway. Not cold enough fast enough. You might be making it harder on yourself... just set the temperature of the cryostat for the appropriate temperature of whatever tissue youre cutting, and you should have no need for a crutch, whether it be fluoroethane or liquid nitrogen.
>
> kisses, flowers and rainbows,
> Bernie Taupin, King of Cryomicrotomy, Esq.
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern..edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 65, Issue 17
> ****************************************
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