[Histonet] Eosinophilic granulocytes

[Jodie Robertson]

Chromotrope 2R also works well.


Jodie Robertson, HT (ASCP) QIHC
Pathology Sciences Medical Group
Histology Day Supervisor
183 E. 8th Ave.
Chico, CA  95926
530-891-6244 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Pritchard, Michele
Sent: Tuesday, April 07, 2009 7:32 AM
To: Bruijntjes, J.P. (Joost); Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Eosinophilic granulocytes

Good morning:

I have good luck by simply using haematoxylin and eosin staining....the
eosinophils pop right out by virtue of their bright pink/red granules!
When used in conjunction with nuclear morphology, one can, with
confidence, call these cells eosinophils.  

I have never performed IHC for eosinophils, but just did a quick search
and found this helpful website.  I am sure there are more websites like
this, but this one will certainly get you started.

http://www.antibodybeyond.com/reviews/cell-markers/eosinophil-marker.htm

Best of luck

-->mtp

Michele T. Pritchard, Ph.D.
Research Associate
Nagy Laboratory
Department of Pathobiology/NE40
Lerner Research Institute
Cleveland Clinic
9500 Euclid Avenue
Cleveland, OH 44195
 
phone:  216.444.8613
fax:  216.636.1493
 
email:  pritchm <@t> ccf.org
 
Lab location:
Lerner Research Institute
NE4-214
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Bruijntjes, J.P. (Joost)
Sent: Tuesday, April 07, 2009 10:14 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Eosinophilic granulocytes

Hi

 

Is anyone of you aware of a marker/method to stain eosinophilic
granulocytes in mouse lung tissue which is formalin fixed and paraffin
embedded?

 

Thanks

Joost Bruijntjes

TNO Quality of Life

Zeist

Holland

 

TNO.NL <http://www.tno.nl/> 

Joost Bruijntjes

T +31 30 694 44 80
F +31 30 694 49 86
E joost.bruijntjes <@t> tno.nl <mailto:joost.bruijntjes <@t> tno.nl> 

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