[Histonet] Eosinophilic granulocytes

[Pritchard, Michele]

Good morning:

I have good luck by simply using haematoxylin and eosin staining....the
eosinophils pop right out by virtue of their bright pink/red granules!
When used in conjunction with nuclear morphology, one can, with
confidence, call these cells eosinophils.  

I have never performed IHC for eosinophils, but just did a quick search
and found this helpful website.  I am sure there are more websites like
this, but this one will certainly get you started.

http://www.antibodybeyond.com/reviews/cell-markers/eosinophil-marker.htm

Best of luck

-->mtp

Michele T. Pritchard, Ph.D.
Research Associate
Nagy Laboratory
Department of Pathobiology/NE40
Lerner Research Institute
Cleveland Clinic
9500 Euclid Avenue
Cleveland, OH 44195
 
phone:  216.444.8613
fax:  216.636.1493
 
email:  pritchm <@t> ccf.org
 
Lab location:
Lerner Research Institute
NE4-214
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Bruijntjes, J.P. (Joost)
Sent: Tuesday, April 07, 2009 10:14 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Eosinophilic granulocytes

Hi

 

Is anyone of you aware of a marker/method to stain eosinophilic
granulocytes in mouse lung tissue which is formalin fixed and paraffin
embedded?

 

Thanks

Joost Bruijntjes

TNO Quality of Life

Zeist

Holland

 

TNO.NL <http://www.tno.nl/> 

Joost Bruijntjes

T +31 30 694 44 80
F +31 30 694 49 86
E joost.bruijntjes <@t> tno.nl <mailto:joost.bruijntjes <@t> tno.nl> 

Disclaimer <http://www.tno.nl/tno/email/> 

 

This e-mail and its contents are subject to the DISCLAIMER at
http://www.tno.nl/disclaimer/email.html
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


===================================

P Please consider the environment before printing this e-mail

Cleveland Clinic is ranked one of the top hospitals
in America by U.S. News & World Report (2008).  
Visit us online at http://www.clevelandclinic.org for
a complete listing of our services, staff and
locations.


Confidentiality Note:  This message is intended for use
only by the individual or entity to which it is addressed
and may contain information that is privileged,
confidential, and exempt from disclosure under applicable
law.  If the reader of this message is not the intended
recipient or the employee or agent responsible for
delivering the message to the intended recipient, you are
hereby notified that any dissemination, distribution or
copying of this communication is strictly prohibited.  If
you have received this communication in error,  please
contact the sender immediately and destroy the material in
its entirety, whether electronic or hard copy.  Thank you.