[Histonet] gi microwave processing

[Tony Henwood]

Nancy,

Best advice I can offer is to ensure as much fixation as you can.
We start with 30 minutes at 30oC and the ramp it up to 40oC for 30-60
minutes, rinse in 70% ethanol (5min)then continue microwave processing
with isopropanol and wax. You may also consider decreasing the
processing temperatures.

GI biopsies being quite small do not need over-the-top processing, but
in my experience, fixation is still the key.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
MAGODLEY <@t> aol.com
Sent: Tuesday, 7 April 2009 10:11 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] gi microwave processing


I am starting a gi lab and using a microwave  processor for my first
time.  
Any suggestions?   SHUR Wave  processor.  Thanks, Nancy
**************A Good Credit Score is 700 or Above. See yours in just 2
easy 
steps! 
(http://pr.atwola.com/promoclk/100126575x1221621488x1201450096/aol?redir
=http:%2F%2Fwww.freecreditreport.com%2Fpm%2Fdefault.aspx%3Fsc%3D668072%2
6hmpgID
%3D62%26bcd%3DAprilfooterNO62)
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

*********************************************************************
This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been
virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.
**********************************************************************