[Histonet] "FREEZY" spray

[Amspacher, September]

I was always taught that you never use it when doing frozens because
spraying like that into the chamber will aerosolize any particles in the
chamber i.e.- TB, as for when cutting only when you have too, it can
crack the blocks and stuff like that. 
September HT 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Senn,
Amy R
Sent: Wednesday, April 01, 2009 11:24 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] "FREEZY" spray

I was taught that freeze spray shouldn't be used - ever - on any tissue
at all.

We were told that it damages the tissue.  Am I wrong??

 

We don't even use it when doing frozens - we have a different system now
that extracts all the warmth and freezes the tissue completely so that
we don't need the spray at all.

 

Thanks for everyone's input on this! It will answer a lot of questions
in our lab!

Amy HT

 

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