[Histonet] Calcium salts

Wed Apr 1 07:55:27 CDT 2009

Sodium thiosulfate removes excess silver and has been used in the Von Kossa
technique for years as well as most,if not all silver stains we do.
Bernice

Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL 
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tony Henwood
Sent: Tuesday, March 31, 2009 5:27 PM
To: Anthony Reilly; histonet
Subject: RE: [Histonet] Calcium salts

Tony,

I have been lead to believe that after von Kossa staining, Silver
carbonate dissolves in sodium thiosulphate and cannot be demonstrated
with von Kossa's technique. Though I have not confirmed this

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Anthony
Reilly
Sent: Tuesday, 31 March 2009 4:33 PM
To: histonet
Subject: [Histonet] Calcium salts

Hello

I am trying to differentiate Calcium Phosphate from Calcium Carbonate
which both stain with the von Kossa method.  Is there a pretreatment for
which only one is soluble that could be used to differentiate the 2
components.

thanks

Tony Reilly

Chief Scientist
Anatomical Pathology
Pathology Queensland
Level 1, Building 15
Princess Alexandra Hospital
Ipswich Rd, 
Woolloongabba Q 4102
Australia
Ph: 07 32402412
Fax:07 32402930
tony_reilly <@t> health.qld.gov.au

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