[Histonet] Effects of Cytocool on IHC

[Rene J Buesa]

That is another "urban legend", spray if you need, no effect on IHC (or anything else) whatsoever.
René J.

--- On Tue, 3/31/09, Sebree Linda A <LSebree <@t> uwhealth.org> wrote:

From: Sebree Linda A <LSebree <@t> uwhealth.org>
Subject: RE: [Histonet] Effects of Cytocool on IHC
To: "Leslie McCormack" <aeshnidae <@t> hotmail.com>, histonet <@t> lists.utsouthwestern.edu
Date: Tuesday, March 31, 2009, 5:04 PM

I have heard not to use freezing spray on blocks/sections that are
destined for IHC but I never heard the reasoning behind this warning.
I've always steered clear of it when cutting for IHC and instead will
bury my block in the frost of our freezer until its good and cold.
Works fine.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Leslie
McCormack
Sent: Tuesday, March 31, 2009 2:26 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Effects of Cytocool on IHC



In our lab many of the histotechs use Cytocool when cutting paraffin
blocks.  Has anyone heard of this having a negative effect on IHC?  

 

Leslie McCormack

Baptist Medical Center

Jacksonville, FL 32207

_________________________________________________________________
Express your personality in color! Preview and select themes for
Hotmail(r).
http://www.windowslive-hotmail.com/LearnMore/personalize.aspx?ocid=TXT_M
SGTX_WL_HM_express_032009#colortheme____________________________________
___________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet