[Histonet] which one first - antigen retrieval or endoperoxidase block?

[Whitaker, Bonnie]

Hi Everyone,

I have always done the quench 1st if I do it manually.  If it is automated,
then unless you have a newer instrument with onboard retrieval, you don't
have a choice but to quench after the retrieval step (and may not have a
choice then depending upon the system and how "open" it is).

Bonnie Whitaker

Clinical Histology Manager
Ohio State University Medical Center
N308B Doan Hall
410 W. 10th Ave.
Columbus, OH  43210

Bonnie.Whitaker <@t> osumc.edu

phone 614.293.5048
fax 614.293.7273
pager 614.346.5013

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Greg Dobbin
Sent: Thursday, September 25, 2008 3:12 PM
To: PMonfils <@t> Lifespan.org; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] which one first - antigen retrieval or endoperoxidase
block?

I'd say it doesn't matter which is 1st, except my inclination is always to do
the quench step 1st because the tissues seem a little less delicate before
retreival than after.
Greg

Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE    C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

"And in the end it's not the years in your life that count. It's the life in
your years." 
- Abraham Lincoln


>>> "Monfils, Paul" <PMonfils <@t> Lifespan.org> 9/25/2008 3:26:01 PM >>>
When doing immunoperoxidase staining on a tissue that requires antigen
retrieval, which do you do first - the antigen retrieval or the endogenous
peroxidase blocking?  Or does it matter?
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