[Histonet] which one first - antigen retrieval or endo peroxidase block?

[Greg Dobbin]

I'd say it doesn't matter which is 1st, except my inclination is always
to do the quench step 1st because the tissues seem a little less
delicate before retreival than after.
Greg

Greg Dobbin, R.T.
Chief Technologist, Anatomic Pathology
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE    C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

"And in the end it's not the years in your life
that count. It's the life in your years." 
- Abraham Lincoln


>>> "Monfils, Paul" <PMonfils <@t> Lifespan.org> 9/25/2008 3:26:01 PM >>>
When doing immunoperoxidase staining on a tissue that requires antigen
retrieval, which do you do first - the antigen retrieval or the
endogenous peroxidase blocking?  Or does it matter?
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