AW: [Histonet] Bone Marrow Biopsy Fixation
Gudrun Lang
gu.lang <@t> gmx.at
Thu Sep 25 01:52:34 CDT 2008
Angela,
we use NBF for at least 16 hours, the next day after receiving they are put
in decal (mixture of formic acid and hydrochloric acid with formaldehyd) for
7-8 hours. Our biopsies are about 3-4 mm thick.
After acid decalcification enzyme-stains are not possible any more and the
iron-stain could be false negative. But we have good experiences with the
IHC and had even an improvement with some markers compared with EDTA-decal.
Gudrun Lang
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754
-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Angela R.
Murray
Gesendet: Mittwoch, 24. September 2008 19:40
An: Histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Bone Marrow Biopsy Fixation
Have you fixed bone marrow biopsies in formaling and if so for how long
and what decal solution is best?
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