[Histonet] FFPE mouse tissue problem

[Judy Strauss]

Good Morning,

For the first time I find myself working with decalcified mouse tissue and
all is not well.  Bone is fine but the muscle tissue in the block is
"crispy" and shreds when sectioned.  I do not know if the tissue is
over/under fixed or over/under processed.  I would like to know why mouse
tissue behaves so differently from rat.  I would appreciate a protocol
(along with helpful hints) for embedding decalcified mouse hind limbs in
paraffin.  

Thanks in advance,


Judith Strauss
SUNY Upstate Medical University
Department of Orthopedic Surgery
IHP   room 3118
505 Irving Avenue
Syracuse, NY  13120

phone: (315) 464-9960
fax:  (315) 464-6638