[Histonet] RE: [IHCRG] MAOB IHC on either frozen or FFPE sections

Swain, Frances L SwainFrancesL <@t> uams.edu
Fri Oct 24 11:51:26 CDT 2008


Hi bert ifyou will look at the image for the antibody for IHC of MAOB you
will see at the bottom that the antibody demonstrated was at a concentration
of 3 ug/ml

 

Frances L. Swain HT(ASCP) A. A. S.

Special Procedures Technician

Department of Orthopaedic Surgery

Center for Orthopaedic Research

Barton Research Building 2R28

4301 West Markham Street

Little Rock AR 72205

(501) 686-8739 PHONE

(501) 686-8987 FAX

swainfrancesl <@t> uams.edu email

________________________________

From: ihcrg <@t> googlegroups.com [mailto:ihcrg <@t> googlegroups.com] On Behalf Of
Connolly, Brett M
Sent: Friday, October 24, 2008 10:41 AM
To: ihcrg <@t> googlegroups.com; histonet <@t> lists.utsouthwestern.edu
Subject: [IHCRG] MAOB IHC on either frozen or FFPE sections

 

Has anyone done it? I have an antibody from Abnova. They illustrate staining
of FFPE kidney at 3ug/mL, but when I asked what the stock Ab concentration is
they say they never determined that. Go figure? I'm looking for a starting
point dilution.

Thanks, 
Brett 

Brett M. Connolly, Ph.D. 
Research Fellow, Imaging Research 
Merck & Co., Inc. 
PO Box 4, WP-44K 
West Point, PA 19486 
PH 215-652-2501 fax. 215-993-6803 
e-mail. brett_connolly <@t> merck.com 

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