[Histonet] PAS with Glycogen Digestion - Solution Not Working

[McKnight, Tanisha]

Hi All:
 
I am in the process of working up a PAS Staining protocol. The PAS stain
is working fine, but I am unable to get my tissue to digest. I am using
.5g of Diastase in 50ml of DI water. I tried heating the solution in a
water bath for 30 min, 1 hour and 1.5 hours and it still looks the same
as the one without digestion. Could I be using the wrong Diastase? If
so, does anyone have any suggestions for a new product?
 
Thanks,
 
Tanisha Neely, HT(ASCP)
AP-Histology/Specimen Management
 <mailto:tanisha.mcknight <@t> covance.com>  




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