[Histonet] restarting DAB reaction

Emily Sours talulahgosh <@t> gmail.com
Mon Oct 13 12:17:49 CDT 2008


Has anyone ever tried to continue a DAB reaction after the slides have been
washed and coverslipped?
I can't think of a reason why this wouldn't work, since we just wash the
slides in PBS and water after the DAB step.
We use a vectastain kit for this--would we need to add more of the ABC
reagent (after removing the coverslips and washing in PBS) and then do DAB?

Emily
-- 
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