[Histonet] section thickness

[Weems, Joyce]

We routinely cut at 4 um, 3 um for lymph nodes, bone marrows, and needle
biopsies.

Joyce

Joyce Weems
Pathology Manager
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
Please note new phone and fax numbers
678-843-7376 - Phone
678-843-7831 - Fax

 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of LINDA
MARGRAF
Sent: Thursday, October 09, 2008 1:50 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] section thickness


Histonetters:
Here's a query from Janet that she couldn't get to post to the list.
Please respond to the list so she'll see the response. Thanks.
I would like to poll the group regarding routine practices for thickness
of paraffin sections. I am particularly interested in brain, spinal
cord, lymph node, tonsil, and renal biopsy Most references range from
3-6 um for routine tissues with 4-5um being most prevalent. What do you
use? Which tissues do you cut at a different thickness routinely? 
I am not referring to special stains as the protocol would prescribe the
thickness.
Thanks for your help.
Janet Keeping A.R.T., B. Voc. Ed
Instructor
Medical Laboratory Sciences
 
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