[Histonet] Decalcification prior to Alizarine red or von Kossa possible?

[Alexandra Meinl]

Hello all,

I have to stain some specimens with bone formation in soft tissue. They want
me to do Alizarine red or Kossa stain.
But there are some heavily mineralized nodules in the tissue, I think they
can't be cut nicely without some kind of decalcification.
How should I treat these samples? Is there a way to decalcify them without
negative effects on the calcium stain?

Thanks in advance!

Alexandra