[Histonet] Help with protocols

Histonet Alias histonetalias <@t> gmail.com
Fri Nov 28 13:58:48 CST 2008

If the CC1 standard 32 with amp, 42 degrees does not work then try using
protease 1 4-6 minutes on it.

On Fri, Nov 28, 2008 at 12:35 PM, Lena Spencer <lenaspencer <@t> insightbb.com>wrote:

> Hi All:
> I have been working up the PMS2 and MLH2 mismatched repair genes and I am
> not satisfied with my results.  I am using the Cell Marque antibodies and
> the Ventana XT using CC1, mild, standard and I have tried the antibodies
> with block and amplification, and antibody times from 32 min- 1 hour and
> the
> stain is not satisfactory.  Anyone who has these antibodies in their lab
> and
> would like to share your  protocols,  I would be grateful for your help.
> The MSH2 and MSH6 are working great.
> Lena Spencer
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The Unknown HT(ASCP)

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