[Histonet] Help with protocols

Lena Spencer lenaspencer <@t> insightbb.com
Fri Nov 28 11:35:37 CST 2008

Hi All:

I have been working up the PMS2 and MLH2 mismatched repair genes and I am
not satisfied with my results.  I am using the Cell Marque antibodies and
the Ventana XT using CC1, mild, standard and I have tried the antibodies
with block and amplification, and antibody times from 32 min- 1 hour and the
stain is not satisfactory.  Anyone who has these antibodies in their lab and
would like to share your  protocols,  I would be grateful for your help.
The MSH2 and MSH6 are working great.

Lena Spencer

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