[Histonet] noradrenaline staining

Dr. med. Frauke Neff nefff <@t> staff.uni-marburg.de
Tue Nov 25 11:33:35 CST 2008

Dear Netters,
one researcher wants me to do an immunohistochemistry stain of rat brains for
noradrenaline. The data sheet mentioned that the tissue should have been fixed
with glutaraldehyde but I just got FFPE and a few snap frozen samples. Is it
possible to thaw the frozen samples and fix them with glutaraldehyde or does it
produce lots of artifacts? Does anyone have an idea how to go on with the FFPE
samples? Does anyone know an alternative stain for noradrenaline? I read
something about masson-goldner stain, but I don't know if it is going to work
on brains.

Thanks in advance,


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