[Histonet] Anti-fluorophore antibodies (for MOM work)

Mikael Niku mikael.niku <@t> helsinki.fi
Fri Nov 21 06:41:47 CST 2008

Dear Histonetters,

I'd like to ask for recommendations for anti-fluorophore antibodies. I'm
planning to use a fluorophore-labelled primary antibody for mouse-on-mouse
IHC. This is then detected using a labelled anti-rabbitIg (or whatever host
it is) ab.

Apparently Dako has discontinued the unlabelled rabbit anti-FITC antibody
that I understand used to be popular in these applications, and I was told
the remaining HRP version doesn't work well for subsequent detection with an
antiIg ab.

Has anyone tried the rabbit anti-Alexa488 antibody by Molecular Probes /
Invitrogen? Or are there any other good anti-FITC abs available?

With best regards,
Mikael Niku

 Mikael Niku, PhD, university lecturer            
 University of Helsinki, Division of Nutrition
 URL: mikael.nikunnakki.info

 - What do I think of western civilization?
   I think it would be a good idea!                       

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