AW: [Histonet] Control slide storage

[Edwards, R.E.]

I  feel  that  as  far  as  surgical human samples that were  originally taken for a quick diagnoses( which means  minimal NBF fixation) with a just  H <@t> E,  the first 50-100u into  the  block  is  fine, after  that  who  knows  how  well  it  is  fixed/processed and  this is   where  the  repeat sections are taken for  subsequent  immunostaining, which  could  lead  to  dodgy results; just  a  thought!!

-----Original Message-----
From: Gudrun Lang [mailto:gu.lang <@t> gmx.at] 
Sent: 20 November 2008 18:57
To: 'Edwards, R.E.'
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: AW: AW: [Histonet] Control slide storage

I think the culprit is a "kind of oxidation" the epitopes suffer from. But
it would be interesting, if the fixation is a factor of influence, or if the
composition of the epitope itself makes the difference.
Gudrun

-----Ursprüngliche Nachricht-----
Von: Edwards, R.E. [mailto:ree3 <@t> leicester.ac.uk] 
Gesendet: Donnerstag, 20. November 2008 17:34
An: 'Jackie M O'Connor'; gu.lang <@t> gmx.at
Cc: histonet <@t> lists.utsouthwestern.edu;
histonet-bounces <@t> lists.utsouthwestern.edu
Betreff: RE: AW: [Histonet] Control slide storage

Is  it    quite  as  simple  as  that, I  imagine that many  epitopes might
lose "stainability" if fixation is inadequate or processing poorly
controlled.




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jackie M
O'Connor
Sent: 20 November 2008 15:47
To: gu.lang <@t> gmx.at
Cc: histonet <@t> lists.utsouthwestern.edu;
histonet-bounces <@t> lists.utsouthwestern.edu
Subject: Re: AW: [Histonet] Control slide storage

CD31 for sure.  I do recall most surface markers I used tended to be 
unstable.

Jackie O'



"Gudrun Lang" <gu.lang <@t> gmx.at> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
11/20/2008 09:17 AM
Please respond to
gu.lang <@t> gmx.at


To
<histonet <@t> lists.utsouthwestern.edu>
cc

Subject
AW: [Histonet] Control slide storage






Hi Patsy,
I am interested in the names of this certain epitopes, that loose
stainability after long time storage.

And I'd like to ask all the other listmembers about their experiences with
this issue. Perhaps we can make a list of "dangerous" epitopes.

Regards
Gudrun Lang

Akh Linz, Austria

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Patsy
Ruegg
Gesendet: Donnerstag, 20. November 2008 15:09
An: Cindy.J.Chard-Bergstrom <@t> aphis.usda.gov;
histonet <@t> lists.utsouthwestern.edu
Betreff: RE: [Histonet] Control slide storage

I store all cut paraffin sections for IHC control at 4dc and without 
heating
to melt the paraffin, even with that I have seen some loss of antigenicity
over long periods of time for certain antibodies.

Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
pruegg <@t> ihctech.net
www.ihctech.net
www.ihcrg.org 
 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Cindy.J.Chard-Bergstrom <@t> aphis.usda.gov
Sent: Wednesday, November 19, 2008 2:31 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Control slide storage

The issue was brought up in the lab regarding the storing of IHC control 
slides. What experience has anyone had on the effects of long term storage 

and staining quality. We store the slides non preheated (with the paraffin 

still on them). 


Cindy J Chard-Bergstrom
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