[Histonet] RE: Uneven H&E Staining

Josie Britton JCBRITTON <@t> Cheshire-Med.COM
Wed Nov 19 12:51:18 CST 2008

Sounds to me that your having a de-paraffining problem.  Check your xylene or clearing agent, maybe there is water in them or they are not as clean as they should be.  Are you on a daily rotation/change of staining/de-paraffin reagents?  Maybe, your oven isn't hot enough to melt some paraffin off the slides before de-par!


Josie Britton HT  


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Wednesday, November 19, 2008 9:48 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: AW: [Histonet] RE: Uneven H&E Staining


Are the coplin jars all equally filled? If the wash-jars are partly filled,

the lower slides would be more differentiated than the upper.



-----Ursprüngliche Nachricht-----

Von: histonet-bounces <@t> lists.utsouthwestern.edu

[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Karla


Gesendet: Mittwoch, 19. November 2008 02:34

An: histonet <@t> lists.utsouthwestern.edu

Betreff: [Histonet] RE: Uneven H&E Staining


Recently we have had an issue with uneven H&E staining.  Lately slides that


three sections on one slide, mostly GI biopsies, and the last section

(bottom of 

slide) are paler than the upper two section.  All cut by the same person and


stains were great.  What could be the cause of this?  Also with the quality


formalin.  Can formalin be purchased as "bad" when it takes 6 days to fix


or breasts?  What is the cause? 


Karla Arrington, HT(ASCP)





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