AW: [Histonet] RE: Uneven H&E Staining

Gudrun Lang gu.lang <@t>
Wed Nov 19 08:48:25 CST 2008

Are the coplin jars all equally filled? If the wash-jars are partly filled,
the lower slides would be more differentiated than the upper.

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] Im Auftrag von Karla
Gesendet: Mittwoch, 19. November 2008 02:34
An: histonet <@t>
Betreff: [Histonet] RE: Uneven H&E Staining

Recently we have had an issue with uneven H&E staining.  Lately slides that
three sections on one slide, mostly GI biopsies, and the last section
(bottom of 
slide) are paler than the upper two section.  All cut by the same person and
stains were great.  What could be the cause of this?  Also with the quality
formalin.  Can formalin be purchased as "bad" when it takes 6 days to fix
or breasts?  What is the cause? 
Karla Arrington, HT(ASCP)

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