AW: [Histonet] RE: Uneven H&E Staining

[Gudrun Lang]

Are the coplin jars all equally filled? If the wash-jars are partly filled,
the lower slides would be more differentiated than the upper.
Gudrun

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Karla
Arrington
Gesendet: Mittwoch, 19. November 2008 02:34
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] RE: Uneven H&E Staining

Recently we have had an issue with uneven H&E staining.  Lately slides that
have 
three sections on one slide, mostly GI biopsies, and the last section
(bottom of 
slide) are paler than the upper two section.  All cut by the same person and
the 
stains were great.  What could be the cause of this?  Also with the quality
of 
formalin.  Can formalin be purchased as "bad" when it takes 6 days to fix
placenta 
or breasts?  What is the cause? 
 
Karla Arrington, HT(ASCP)


      
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