[Histonet] Antibody protocol

Weber, Susan (VHACLE) Susan.Weber2 <@t> va.gov
Wed Nov 19 09:54:20 CST 2008

We are using the ultraView DAB from Ventana and the same HSA from Cell
Marque. Here is the protocol we are using. Depar as usual; CC1 Standard;
HSA incubation at 37C for 32 min; Counterstain Heme 4 min; Post
Counterstain Bluing for 4 min. we have found it to be a reliable stain
and very clean and crisp. 

Susan M Weber HT(ASCP)
Histology Supervisor
Louis Stokes Cleveland VA Medical Center
10701 East Blvd
Cleveland, Ohio 44106
(216) 791-3800 X6154
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jesus
Sent: Tuesday, November 18, 2008 12:37 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Antibody protocol

Needing some help with the staining protocol for  HSA (Hepatocyte
Specific Antigen) clone OCH1E5, made by Cell marquee to be ran on the
Ventana LT/Benchmark platform.. Would anyone be willing to share
protocol information with us, as well as staining methodology.  Thank
you for the help.  


Jesus A Ellin  HT/PA  ASCP

Department of Pathology/Histology

Yuma Regional Medical Center

2400 South Ave A

Yuma, AZ  85364 - 7170

( Office:  (928) 336-1743

(    Fax:  (928) 336-7319

*    Email: jellin <@t> yumaregional.org 

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