[Histonet] HistoClear question

[Rene J Buesa]

Andi:
Histoclear (in all its "variants" and names, like Histolene) is a d-Limonene  containing substance known to having incompatibility problems with mounting media, specially those toluene containing and has to be used with the proprietary mounting medium brand Histomount that is xylene based.
Besides that, Histoclear is notorious for fading hematoxylin, and is not as good as xylene for dewaxing sections before IHC. Has a strong odor and has been reported as hardening brain, liver and spleen. Has caused skin allergic reactions and headaches.
René J.

--- On Tue, 11/18/08, Andrea Grantham <algranth <@t> u.arizona.edu> wrote:

From: Andrea Grantham <algranth <@t> u.arizona.edu>
Subject: [Histonet] HistoClear question
To: histonet <@t> lists.utsouthwestern.edu
Date: Tuesday, November 18, 2008, 11:23 AM

A student in my lab is doing some work in another lab here and having some
problems with coverslipping their H&E sections. They are using HistoClear 1X
as a clearing agent and Cytoseal mounting medium. From looking at the
coverslipped slides I'm guessing that these two products are not compatible.
The mounting medium is sort of clumping up under coverslips and has a hazy
appearance. Yes, I did also find a bit of water in their HistoClear as well.
Does anybody else have any experience with HistoClear 1X and/or does anybody
know what mounting medium is good to use with the product? I suggested that they
call the company who manufactures HistoClear but I don't know if they have
done that yet.

Thanks,

Andi Grantham
.....................................................................
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