[Histonet] HistoClear question

[Jo Dee Fish]

Dear Andrea,

We stopped using the xylene substitute when my new co-worker started here.
She doesn't like change and since she used only xylene at her last job, I
compromised and switched back to using the real stuff.  You have to choose
your battles and since she would have set up additional stations to include
the xylene anyway, I gave in.

I used to use the mounting medium from ThermoElectron, called Shandon Xylene
Substitute Mountant.  It was nicely miscible with the histoclear.  I don't
know the new catalog number since they have merged so many times with other
companies.  I guess you could search the Fisher Scientific website.

Good luck,
Jo Dee


~~Jo Dee Fish~~
Senior Research Technologist
The J. David Gladstone Institutes
Co-manager Histology and Microscopy Core
 
Telephone: (415) 734-2567
Fax: (415) 355-0824
E-mail: jfish <@t> gladstone.ucsf.edu
 
Mailing address:
The J. David Gladstone Institutes
1650 Owens Street
San Francisco, CA 94158

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Andrea
Grantham
Sent: Tuesday, November 18, 2008 8:23 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] HistoClear question

A student in my lab is doing some work in another lab here and having some
problems with coverslipping their H&E sections. They are using HistoClear 1X
as a clearing agent and Cytoseal mounting medium. From looking at the
coverslipped slides I'm guessing that these two products are not compatible.
The mounting medium is sort of clumping up under coverslips and has a hazy
appearance. Yes, I did also find a bit of water in their HistoClear as well.
Does anybody else have any experience with HistoClear 1X and/or does anybody
know what mounting medium is good to use with the product? I suggested that
they call the company who manufactures HistoClear but I don't know if they
have done that yet.

Thanks,

Andi Grantham
.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
: (office:  AHSC 4212)          P.O. Box 245044                     :
: (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
: (FAX:  520-626-2097)          (email:  algranth <@t> u.arizona.edu)       :
:...................................................................:
           http://www.cba.arizona.edu/histology-lab.html


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