[Histonet] Oil Red O despair

[Derek Papalegis]

I have had much better luck staining liver samples with Sudan Black B 
rather than with Oil Red O. I suggest giving that a try.

Derek Papalegis HT (ASCP)
Senior Histology Technologist
Division of Laboratory Animal Medicine
Tufts University 
136 Harrison Avenue
Boston, MA 02111
phone: 617 636-2971
fax: 617 636-8354



pam johnson wrote:
> Michele,
>
> I am having the same issues. I cannot get a good stain. I am getting alot of non-specific staining. I am using the kit from PolyScientific. I am using mouse tissue. I have never had so many problems with an Oil Red O. It is such a simple stain.
>
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> -----Original Message-----
> From: Michele Wich <mwich <@t> 7thwavelabs.com>
> To: histonet <@t> lists.utsouthwestern.edu
> Sent: Mon, 17 Nov 2008 11:56 am
> Subject: [Histonet] Oil Red O despair
>
>
>
> I'm wondering what are the pros (or cons) of using the propylene glycol
> version of Oil Red O over the isopropanol method. Is one more suited to
> a specific application? 
>
> I'm trying to stain a frozen section of liver, which one would suspect
> would be loaded with fat, and have thus far been unsuccessful using the
> propylene glycol Oil Red O.
>
> Is there something obvious that I'm missing here? I'm new to
> cryosectioning...perhaps I did something wrong in the cryostat. I fixed
> my sections in NBF before staining.
>
> Please help. I'm feeling like a pathetic excuse for a histotech. Any
> advice is greatly appreciated.
>
>
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