[Histonet] Oil Red O despair

pam johnson jhnspam <@t> aol.com
Mon Nov 17 12:03:18 CST 2008


I am having the same issues. I cannot get a good stain. I am getting alot of non-specific staining. I am using the kit from PolyScientific. I am using mouse tissue. I have never had so many problems with an Oil Red O. It is such a simple stain.

-----Original Message-----
From: Michele Wich <mwich <@t> 7thwavelabs.com>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Mon, 17 Nov 2008 11:56 am
Subject: [Histonet] Oil Red O despair

I'm wondering what are the pros (or cons) of using the propylene glycol
version of Oil Red O over the isopropanol method. Is one more suited to
a specific application? 

I'm trying to stain a frozen section of liver, which one would suspect
would be loaded with fat, and have thus far been unsuccessful using the
propylene glycol Oil Red O.

Is there something obvious that I'm missing here? I'm new to
cryosectioning...perhaps I did something wrong in the cryostat. I fixed
my sections in NBF before staining.

Please help. I'm feeling like a pathetic excuse for a histotech. Any
advice is greatly appreciated.

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