[Histonet] kappa/lambda and..

[Tony Henwood]

You are probably correct.
Remember it would only be one of the variables that affects this localisation.
A similar phenomenon occurs with yolk sac tumours stained for alpha fetoprotein. Short fixation often give a heavy background where as if the tissue is allowed to "stew" in formalin for several weeks a lot of this staining disappears.
We found this with control blocks that were prepared several months after the initial diagnostic stain.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: Gudrun Lang [mailto:gu.lang <@t> gmx.at] 
Sent: Friday, 14 November 2008 2:23 AM
To: Tony Henwood
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: AW: [Histonet] kappa/lambda and..


What duration would you see as "extensive" fixation?
18-24 hours? Or beyond?
Regards
Gudrun

-----Ursprüngliche Nachricht-----
Von: Tony Henwood [mailto:AnthonyH <@t> chw.edu.au] 
Gesendet: Donnerstag, 13. November 2008 00:16
An: gu.lang <@t> gmx.at; histonet <@t> lists.utsouthwestern.edu
Betreff: RE: [Histonet] kappa/lambda and..

Yep,

Extensive NBF fixation tends to rinse out the extra-cellular immunoglobulins and so decreases its contribution to the background staining.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Thursday, 13 November 2008 5:00 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] kappa/lambda and..


Thank you for your answers. An additional question: Can someone confirm, that underfixed tissue shows false positive kappa and lambdastaining?

Bye
Gudrun

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Gudrun Lang
Gesendet: Mittwoch, 12. November 2008 16:45
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] experiences with kappa/lambda

Hi listmembers,

Please tell me about your experiences with the IHC-demonstration of kappa and lambda on FFPE-sections.

We have some troubles about inconsistend staining and therefore false results about the monoclonality of tumors.

Bye

Gudrun

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