AW: [Histonet] kappa/lambda and..

[Gudrun Lang]

What duration would you see as "extensive" fixation?
18-24 hours? Or beyond?
Regards
Gudrun

-----Ursprüngliche Nachricht-----
Von: Tony Henwood [mailto:AnthonyH <@t> chw.edu.au] 
Gesendet: Donnerstag, 13. November 2008 00:16
An: gu.lang <@t> gmx.at; histonet <@t> lists.utsouthwestern.edu
Betreff: RE: [Histonet] kappa/lambda and..

Yep,

Extensive NBF fixation tends to rinse out the extra-cellular immunoglobulins
and so decreases its contribution to the background staining.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Thursday, 13 November 2008 5:00 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] kappa/lambda and..


Thank you for your answers. An additional question: Can someone confirm,
that underfixed tissue shows false positive kappa and lambdastaining?

Bye
Gudrun

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Gudrun
Lang
Gesendet: Mittwoch, 12. November 2008 16:45
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] experiences with kappa/lambda

Hi listmembers,

Please tell me about your experiences with the IHC-demonstration of kappa
and lambda on FFPE-sections.

We have some troubles about inconsistend staining and therefore false
results about the monoclonality of tumors.

Bye

Gudrun

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