AW: [Histonet] kappa/lambda and..

Gudrun Lang gu.lang <@t>
Thu Nov 13 09:23:13 CST 2008

What duration would you see as "extensive" fixation?
18-24 hours? Or beyond?

-----Ursprüngliche Nachricht-----
Von: Tony Henwood [mailto:AnthonyH <@t>] 
Gesendet: Donnerstag, 13. November 2008 00:16
An: gu.lang <@t>; histonet <@t>
Betreff: RE: [Histonet] kappa/lambda and..


Extensive NBF fixation tends to rinse out the extra-cellular immunoglobulins
and so decreases its contribution to the background staining.


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] On Behalf Of Gudrun Lang
Sent: Thursday, 13 November 2008 5:00 AM
To: histonet <@t>
Subject: [Histonet] kappa/lambda and..

Thank you for your answers. An additional question: Can someone confirm,
that underfixed tissue shows false positive kappa and lambdastaining?


-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] Im Auftrag von Gudrun
Gesendet: Mittwoch, 12. November 2008 16:45
An: histonet <@t>
Betreff: [Histonet] experiences with kappa/lambda

Hi listmembers,

Please tell me about your experiences with the IHC-demonstration of kappa
and lambda on FFPE-sections.

We have some troubles about inconsistend staining and therefore false
results about the monoclonality of tumors.



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