AW: [Histonet] kappa/lambda and..

Gudrun Lang gu.lang <@t> gmx.at
Thu Nov 13 09:23:13 CST 2008


What duration would you see as "extensive" fixation?
18-24 hours? Or beyond?
Regards
Gudrun

-----Ursprüngliche Nachricht-----
Von: Tony Henwood [mailto:AnthonyH <@t> chw.edu.au] 
Gesendet: Donnerstag, 13. November 2008 00:16
An: gu.lang <@t> gmx.at; histonet <@t> lists.utsouthwestern.edu
Betreff: RE: [Histonet] kappa/lambda and..

Yep,

Extensive NBF fixation tends to rinse out the extra-cellular immunoglobulins
and so decreases its contribution to the background staining.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Thursday, 13 November 2008 5:00 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] kappa/lambda and..


Thank you for your answers. An additional question: Can someone confirm,
that underfixed tissue shows false positive kappa and lambdastaining?

Bye
Gudrun

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Gudrun
Lang
Gesendet: Mittwoch, 12. November 2008 16:45
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] experiences with kappa/lambda

Hi listmembers,

Please tell me about your experiences with the IHC-demonstration of kappa
and lambda on FFPE-sections.

We have some troubles about inconsistend staining and therefore false
results about the monoclonality of tumors.

Bye

Gudrun

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

*********************************************************************
This email and any files transmitted with it are confidential and intended
solely for the use of the individual or entity to whom they are addressed.
If you are not the intended recipient, please delete it and notify the
sender.

Views expressed in this message and any attachments are those of the
individual sender, and are not necessarily the views of The Children's
Hospital at Westmead

This note also confirms that this email message has been
virus scanned and although no computer viruses were detected, The Childrens
Hospital at Westmead accepts no liability for any consequential damage
resulting from email containing computer viruses.
**********************************************************************




More information about the Histonet mailing list