AW: [Histonet] Fatty tissue

Angela Bitting akbitting <@t>
Thu Nov 6 11:03:18 CST 2008

We've done the same. Additional time in Xylene and paraffin made a huge

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 
No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!

>>> "Gudrun Lang" <gu.lang <@t>> 11/6/2008 10:42 AM >>>
We have made some changes to our regular VIP-protocol (13 hours):
time in 100%ethanol, in shell sol (=Xylen-substitute) and paraffin to
This led to an improvement of paraffin-infiltration and

Some blocks can be improved by letting them sit in melted paraffin for
a few
hours after the processing.

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> 
[mailto:histonet-bounces <@t>] Im Auftrag von
Dorothy L
Gesendet: Mittwoch, 05. November 2008 21:22
An: histonet <@t> 
Betreff: [Histonet] Fatty tissue

I would like to know how other labs are processing "fatty" tissue,
especially breast for optimal processing?  We have been doing a few
"reprocessing" of these specimens and my pathologist feels this should
not be happening!  Yes, the PA's are, at times, cutting the sections
larger than 3 mm thick, but, at times the tumor is ingrained in the
fatty area and they need to obtain as much of the perimeter as they
Does anyone agitate or heat fixate their specimens prior to
Would appreciate any feedback on this age old dilemma.
Also, I only heard back from one person on the recycling issue.  Any
input would be appreciated as to savings incurred and a still vs.
This e-mail and any files transmitted with it are confidential and are
intended solely for the use of the individual or entity to whom they
addressed. If you are not the intended recipient or the individual
responsible for delivering the e-mail to the intended recipient, please
advised that you have received this e-mail in error and that any use,
dissemination, forwarding, printing, or copying of this e-mail is
If you have received this e-mail in error, please immediately notify
HealthPartners Support Center by telephone at (952) 967-6600. You will
reimbursed for reasonable costs incurred in notifying us.
Histonet mailing list
Histonet <@t> 

Histonet mailing list
Histonet <@t>

IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.
-------------- next part --------------
FN:Bitting, Angela

More information about the Histonet mailing list