AW: [Histonet] Fatty tissue

[Gudrun Lang]

We have made some changes to our regular VIP-protocol (13 hours): Increasing
time in 100%ethanol, in shell sol (=Xylen-substitute) and paraffin to 16
hours.
This led to an improvement of paraffin-infiltration and sectioning-qualitiy.

Some blocks can be improved by letting them sit in melted paraffin for a few
hours after the processing.
Gudrun

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Webb,
Dorothy L
Gesendet: Mittwoch, 05. November 2008 21:22
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Fatty tissue

I would like to know how other labs are processing "fatty" tissue,
especially breast for optimal processing?  We have been doing a few
"reprocessing" of these specimens and my pathologist feels this should
not be happening!  Yes, the PA's are, at times, cutting the sections
larger than 3 mm thick, but, at times the tumor is ingrained in the
fatty area and they need to obtain as much of the perimeter as they can.
Does anyone agitate or heat fixate their specimens prior to processing?
Would appreciate any feedback on this age old dilemma.
Also, I only heard back from one person on the recycling issue.  Any
input would be appreciated as to savings incurred and a still vs.
recycler.
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