[Histonet] EIER before HIER??

[Sebree Linda A.]

I believe that when we've had to do this in the past, we've done EIER
followed by HIER because that's the order it occurs on Ventana
instruments.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Woodward, Denise
Sent: Monday, November 03, 2008 3:03 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] EIER before HIER??

Hi folks,

 

I have a new antibody that seems to need both Protease digestion and
Citrate buffer antigen retrieval. Does anyone have any experience with
this double whammy approach and know if it works best to do EIER before
HIER or HIER before EIER.  Perhaps it doesn't matter which is first?

Any comments??

Thanks,

Denise Long Woodward

University of Connecticut

Dept. of Pathobiology and Veterinary Science

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