[Histonet] EIER before HIER??
Sebree Linda A.
LSebree <@t> uwhealth.org
Mon Nov 3 15:36:05 CST 2008
I believe that when we've had to do this in the past, we've done EIER
followed by HIER because that's the order it occurs on Ventana
Linda A. Sebree
University of Wisconsin Hospital & Clinics
600 Highland Ave.
Madison, WI 53792
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Sent: Monday, November 03, 2008 3:03 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] EIER before HIER??
I have a new antibody that seems to need both Protease digestion and
Citrate buffer antigen retrieval. Does anyone have any experience with
this double whammy approach and know if it works best to do EIER before
HIER or HIER before EIER. Perhaps it doesn't matter which is first?
Denise Long Woodward
University of Connecticut
Dept. of Pathobiology and Veterinary Science
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