[Histonet] EIER before HIER??

[Woodward, Denise]

Hi folks,

 

I have a new antibody that seems to need both Protease digestion and
Citrate buffer antigen retrieval. Does anyone have any experience with
this double whammy approach and know if it works best to do EIER before
HIER or HIER before EIER.  Perhaps it doesn't matter which is first?

Any comments??

Thanks,

Denise Long Woodward

University of Connecticut

Dept. of Pathobiology and Veterinary Science