[Histonet] Microwave deparaffinization.

[Anne van Binsbergen]

as per the DAKO PT link procedure we have tried dewaxing slides for IHC
using our standard buffers (with tween) and heating them up to the
recommended temps - works like a charm
ER and PR looking good
i could not believe when reading the 'blurb' that the solution used to
'boil' out the wax would not leave a greasy residue on the slides as they
are removed and cooler air congeals the waxy/buffer on the slides
but i was wrong - try it out
Annie

2008/5/21 Tony Henwood <AnthonyH <@t> chw.edu.au>:

> Sebastian,
> The following article uses a heated detergent solution to dewax and
> rehydrate sections. I have not as yet used it myself but I plan to try it at
> some time.
>
> Lars Falkeholm, Crawford A. Grant, Anders Magnusson, and Eva Möller (2001)
> "Xylene-Free Method for Histological Preparation: A Multicentre Evaluation"
> LABORATORY INVESTIGATION Vol. 81, No. 9, p. 1213-1221.
>
>
>
> Regards
>
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
> Laboratory Manager & Senior Scientist
> The Children's Hospital at Westmead,
> Locked Bag 4001, Westmead, 2145, AUSTRALIA.
> Tel: 612 9845 3306
> Fax: 612 9845 3318
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sebastián Vecchio
> Sent: Tuesday, 20 May 2008 5:36 AM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Microwave deparaffinization.
>
>
> I have been trying to deparafinize section in the lab, but I cannot  get
> the right time and  power for the MW oven, does any one has a protocol for
> this . This method eliminates the uses  of xylol in the processing of
> staining sections. Thanks. Sebastian, Student of Histotechnology, EUTM,
> FMED, UDELAR. _______________________________________________
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-- 
Anne van Binsbergen (Hope)
Abu Dhabi
UAE