[Histonet] ZYMED Histostain Plus Kit on animal tissue

[Gayle Callis]

Kim,

This kit detects (in its broad spectrum) - mouse, rabbit, rat and guinea 
pig.  You are correct about this more universal, multilink secondary.  The 
kit protocol is found on the Invitrogen website with information on what is 
in the secondary spectrum.

Gayle M. Callis
HTL/HT/MT(ASCP)
Bozeman MT 59715

----- Original Message ----- 
From: "Kim Merriam" <kmerriam2003 <@t> yahoo.com>
To: "Mary L Giebel/FS/VCU" <mlgiebel <@t> vcu.edu>; 
<histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, May 14, 2008 11:51 AM
Subject: Re: [Histonet] ZYMED Histostain Plus Kit on animal tissue


I dont have experience with this particular kit from Zymed, but the term 
"broad spectrum" makes me think that it would be similar to a universal 
secondary (that might include anti-mouse and anti-rabbit secondaries) which 
would likely cross-react to your rat tissue. It might be worth giving Zymed 
a call to find out about this kit.
Kim Merriam, MA, HT(ASCP)
Cambridge, MA



----- Original Message ----
From: Mary L Giebel/FS/VCU <mlgiebel <@t> vcu.edu>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Wednesday, May 14, 2008 12:56:51 PM
Subject: [Histonet] ZYMED Histostain Plus Kit on animal tissue

At a client's request, I am using a ZYMED's Broad Spectrum Histostain Plus 
Kit (#85-9843) on rat tissue. I have encountered an unexpected staining 
pattern as well as more background staining than usual. I would be very 
interested in hearing from others who have used this kit on animal tissue. I 
found the question posed in the Histonet archives (8/23/2005), but never 
found an answer posted.
Thank you for your help.
Regards,
Mary Giebel




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